Human sperm proteome: immunodominant sperm surface antigens identified with sera from infertile men and women.
Human sperm proteome: immunodominant sperm surface antigens identified with sera from infertile men and women.
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人类精子蛋白质组：用不育男性和女性的血清鉴定的免疫显性精子表面抗原。
DOI：
10.1095/biolreprod61.1.61
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发表时间：
1999
影响因子：
3.6
通讯作者：
Herr,JC
中科院分区：
文献类型：
--
作者：
Shetty,J;Naaby-Hansen,S;Shibahara,H;Bronson,R;Flickinger,CJ;Herr,JC
本研究的目的是鉴定不育男性和女性血清中抗精子抗体（阿萨）识别的免疫显性精子抗原。采用等电聚焦或非平衡pH梯度电泳，高分辨率双向凝胶电泳分离人精子蛋白。采用免疫印迹法和增强化学发光法（ECL）分析了15例男性不育患者和6例女性不育患者的血清样本，这些血清样本通过免疫珠结合试验（IBT）测定含有阿萨。来自10名经IBT检测为阿萨阴性的生育受试者（5名男性和5名女性）的血清样品用作对照。ECL印迹进行了分析，通过计算机扫描，比较从生育和不育的受试者的血清样品之间的免疫反应性，并确定独特的不育受试者的血清的抗原; 98精子自体和同种抗原蛋白点被识别的血清从不育的男性和女性，但不能从生育的受试者。基于在精子表面用125 I进行的矢量标记，鉴定了6种自身抗原和同种抗原的子集，其可能与抗体介导的不育相关。
The objective of this study was to identify those immunodominant sperm antigens recognized by antisperm antibodies (ASA) in the serum samples of infertile men and women. High-resolution two-dimensional gel electrophoresis was employed to separate human sperm proteins using isoelectric focusing or nonequilibrium pH gradient electrophoresis, followed by PAGE. Serum samples from 15 infertile male subjects and 6 infertile female subjects that contained ASA as assayed by the immunobead binding test (IBT) were analyzed by Western blotting followed by enhanced chemiluminescence (ECL). Serum samples from 10 fertile subjects (5 males and 5 females) that were ASA negative by IBT were used as controls. The ECL blots were analyzed by computer scanning to compare the immunoreactivity between serum samples from fertile and infertile subjects and to identify the antigens unique to the sera of the infertile subjects; 98 sperm auto- and iso-antigenic protein spots were recognized by sera from infertile males and females but not from fertile subjects. Based on vectorial labeling with125I at the sperm surface, a subset of 6 auto- and iso-antigens was identified as possibly relevant to antibody-mediated infertility.