Cytotoxic activities of fish leucocytes
Cytotoxic activities of fish leucocytes
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DOI：
10.1016/j.fsi.2005.03.013
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发表时间：
2006-02-01
影响因子：
4.7
通讯作者：
Nakanishi, T
中科院分区：
文献类型：
--
作者：
Fischer, U;Utke, K;Nakanishi, T
与哺乳动物白细胞一样，鱼类的白细胞能够杀死变异的（例如病毒感染的）细胞和外来的（同种异体或异种）细胞。首次使用同种异体和异种效应/靶细胞系统证明鱼类中存在自然杀伤（NK）样细胞和特异性细胞毒性细胞。除了体内和离体研究之外，使用从斑点叉尾鱼建立的许多不同的长期细胞毒性细胞系进行体外分析也做出了非常重要的贡献。在哺乳动物中，特异性细胞介导的细胞毒性（CMC）作为适应性免疫反应的一部分，需要在效应白细胞和靶细胞上表达的许多关键分子。 CD8(+) T 淋巴细胞仅在其抗原受体 (TCR) 与 MHC I 类与靶细胞的结合肽相匹配时才杀死受感染的细胞。鱼类 TCR、CD8 和 MHC I 类基因同源物以及相关基因的表达模式与相似的功能一致。用于分析特定 CMC 的便捷系统最近才可用于将克隆鱼与同基因或同种异体但 MHC I 类匹配细胞系组合的鱼类。研究表明，NK 细胞和细胞毒性 T (T-c) 细胞均参与杀死病毒感染的 MHC I 类匹配和不匹配的靶细胞。对鱼类这些淋巴细胞亚群的分析才刚刚开始。还有证据表明，不同的病毒蛋白会触发不同的杀伤细胞亚群。本综述进一步讨论了鱼类 CMC 在温度/季节和个体发育方面的发现。 (c) 2005 Elsevier Ltd. 保留所有权利。
Like mammalian leucocytes, white blood cells of fish are able to kill altered (e.g. virus-infected) and foreign (allogeneic or xenogeneic) cells. The existence of natural killer (NK)-like and specific cytotoxic cells in fish was first shown using allogeneic and xenogeneic effector/target cell systems. In addition to in vivo and ex vivo studies, very important contributions were made by in vitro analysis using a number of different long-term cytotoxic cell lines established from channel catfish. In mammals, specific cell-mediated cytotoxicity (CMC) as part of the adaptive immune response requires a number of key molecules expressed on effector leucocytes and target cells. CD8(+) T lymphocytes kill infected cells only, if their antigen receptor (TCR) matches the MHC class I with bound peptide of the target cell. Expression patterns of the fish gene homologues for TCR, CD8 and MHC class I, as well as related genes, are in agreement with similar function. Convenient systems for the analysis of specific CMC have only recently become available for fish with the combination of clonal fish with syngeneic or allogeneic but MHC class I matching cell lines. It was demonstrated that both, NK- and cytotoxic T (T-c) cells are involved in the killing of virus infected MHC class I matching and mismatching target cells. Analysis of these lymphocyte subsets is only starting for fish. There is also evidence that the different viral proteins trigger different subsets of killer cells. This review further discusses findings on fish CMC with regard to temperature/seasons and ontogeny. (c) 2005 Elsevier Ltd. All rights reserved.