An immunopharmacological study of the biphasic allergic skin reaction in mice.
An immunopharmacological study of the biphasic allergic skin reaction in mice.
复制标题
小鼠双相过敏性皮肤反应的免疫药理学研究。
DOI：
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发表时间：
1995
影响因子：
2
通讯作者：
H. Mori
中科院分区：
文献类型：
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作者：
H. Nagai;T. Sakurai;N. Inagaki;H. Mori
在 24 小时前用单克隆 IgE 抗体被动致敏的 ddY、ICR、Balb/c 和 Balb/c-nu/nu 小鼠中诱导双相皮肤反应，在用抗原进行表皮攻击后 1 小时和 24 小时达到峰值（分别为立即相反应：IPR 和晚期反应：LPR）。在缺乏肥大细胞的 WBB6F1-W/Wv 小鼠中，仅观察到 LPR。 IPR 的特点是毛细血管通透性快速增加，而 LPR 的特点是 Balb/c 小鼠皮肤增厚，嗜酸性粒细胞和其他炎症细胞显着浸润。组胺 H1 受体拮抗剂，包括苯海拉明和高氯环利嗪，以及过敏性组胺释放抑制剂曲尼司特和氨来呫诺，明显抑制 IPR，但不抑制 LPR。然而，泼尼松龙和地塞米松对两者都有抑制作用。泼尼松龙还抑制 WBB6F1-W/Wv 小鼠的 LPR。这些结果表明，IgE 抗体依赖性双相皮肤反应由肥大细胞和组胺依赖性 IPR 和肥大细胞非依赖性 LPR 组成。
Biphasic skin reactions, with peaks at 1 and 24 h after epicutaneous challenge with antigen (immediate phase response: IPR and late phase response: LPR, respectively), were induced in ddY, ICR, Balb/c and Balb/c-nu/nu mice passively sensitized with monoclonal IgE antibody 24 h before. In WBB6F1-W/Wv mice, which lack mast cells, only the LPR was observed. The IPR was characterized by a rapid increase in capillary permeability and the LPR by skin thickening with significant infiltration of eosinophils and other inflammatory cells in Balb/c mice. Histamine H1 receptor antagonists, including diphenhydramine and homochlorcyclizine, and the allergic histamine release inhibitors, tranilast and amlexanox, clearly inhibited the IPR, but not the LPR. Prednisolone and dexamethasone, however, inhibited both. Prednisolone also inhibited the LPR in WBB6F1-W/Wv mice. These results indicate that IgE antibody-dependent biphasic skin reactions consist of a mast cell- and histamine-dependent IPR and a mast cell-independent LPR.