ISGF3, THE TRANSCRIPTIONAL ACTIVATOR INDUCED BY INTERFERON-ALPHA, CONSISTS OF MULTIPLE INTERACTING POLYPEPTIDE-CHAINS
ISGF3, THE TRANSCRIPTIONAL ACTIVATOR INDUCED BY INTERFERON-ALPHA, CONSISTS OF MULTIPLE INTERACTING POLYPEPTIDE-CHAINS
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DOI：
10.1073/pnas.87.21.8555
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发表时间：
1990-11-01
影响因子：
11.1
通讯作者：
DARNELL, JE
中科院分区：
文献类型：
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作者：
FU, XY;KESSLER, DS;DARNELL, JE
干扰素刺激基因因子3 （ISGF3）是一种依赖配体的转录激活因子，在对干扰素治疗的反应中，在细胞质中组装，易位到细胞核，并结合一致的DNA位点，即干扰素刺激反应元件。我们纯化了ISGF3并鉴定了其组成蛋白：一个48 kDa的dna结合蛋白和三个更大的多肽（84、91和113 kDa），它们本身不具有dna结合活性。ISGF3的多亚基结构很可能反映了它参与接收配体依赖的信号，转位到细胞核，并与DNA结合以激活转录。
Interferon-stimulated gene factor 3 (ISGF3) is the ligand-dependent transcriptional activator that, in response to interferon treatment, is assembled in the cell cytoplasm, is translocated to the nucleus, and binds the consensus DNA site, the interferon-stimulated response element. We have purified ISGF3 and identified its constituent proteins: a DNA-binding protein of 48 kDa and three larger polypeptides (84, 91, and 113 kDa), which themselves do not have DNA-binding activity. The multisubunit structure of ISGF3 most likely reflects its participation in receiving a ligand-dependent signal, translocating to the nucleus, and binding to DNA to activate transcription.