ANALYSIS OF THE STREPTOMYCES-COELICOLOR SIGE GENE REVEALS THE EXISTENCE OF A SUBFAMILY OF EUBACTERIAL RNA-POLYMERASE SIGMA-FACTORS INVOLVED IN THE REGULATION OF EXTRACYTOPLASMIC FUNCTIONS
ANALYSIS OF THE STREPTOMYCES-COELICOLOR SIGE GENE REVEALS THE EXISTENCE OF A SUBFAMILY OF EUBACTERIAL RNA-POLYMERASE SIGMA-FACTORS INVOLVED IN THE REGULATION OF EXTRACYTOPLASMIC FUNCTIONS
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DOI：
10.1073/pnas.91.16.7573
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发表时间：
1994-08-02
影响因子：
11.1
通讯作者：
BUTTNER, MJ
中科院分区：
文献类型：
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作者：
LONETTO, MA;BROWN, KL;BUTTNER, MJ
sigma（E）是一种RNA聚合酶，其表观M（r）因子为28，000，先前通过其指导天蓝色链霉菌的琼脂酶基因（dagA）的P2启动子转录的能力来鉴定。从纯化的sigma（E）的N-末端序列设计的简并寡核苷酸探针用于分离sigma（E）基因（sigE）。sigma（E）的预测序列与其他七种蛋白质的序列相似性最大：黄色粘球菌CarQ、铜绿假单胞菌AlgU、假单胞菌HrpL、大肠杆菌sigma（E）、真养产碱杆菌CnrH、大肠杆菌E. coli（E）。coli FecI和枯草芽孢杆菌SigX，一种功能未知的蛋白。这八种蛋白质定义了真细菌RNA聚合酶因子的一个亚家族，其与其他sigma因子完全不同，在许多情况下，它们不能通过标准的相似性搜索方法鉴定。现有的信息表明，它们都调节细胞质外的功能，它们作为效应分子响应细胞质外的刺激。A. CnrH似乎是质粒编码的因子。
sigma(E), an RNA polymerase a factor of apparent M(r) 28,000, was previously identified by its ability to direct transcription from the P2 promoter of the agarase gene (dagA) of Streptomyces coelicolor. A degenerate oligonucleotide probe, designed from the N-terminal sequence of purified sigma(E), was used to isolate the sigma(E) gene (sigE). The predicted sequence of sigma(E) shows greatest similarity to sequences of seven other proteins: Myxococcus xanthus CarQ, Pseudomonas aeruginosa AlgU, Pseudomonas syringae HrpL, Escherichia coli sigma(E), Alcaligenes eutrophus CnrH, E. coli FecI, and Bacillus subtilis SigX, a protein of unknown function. These eight proteins define a subfamily of eubacterial RNA polymerase factors sufficiently different from other sigma s that, in many cases, they are not identified by standard similarity searching methods. Available information suggests that all of them regulate extracytoplasmic functions and that they function as effector molecules responding to extracytoplasmic stimuli. A. eutrophus CnrH appears to be a plasmid-encoded factor.