The long intergenic non-protein coding RNA 707 promotes proliferation and metastasis of gastric cancer by interacting with mRNA stabilizing protein HuR
The long intergenic non-protein coding RNA 707 promotes proliferation and metastasis of gastric cancer by interacting with mRNA stabilizing protein HuR
复制标题
长基因间非蛋白编码RNA 707通过与mRNA稳定蛋白HuR相互作用促进胃癌增殖和转移
DOI：
10.1016/j.canlet.2018.11.032
复制
发表时间：
2019-01-01
期刊：
影响因子：
9.7
通讯作者：
De, Wei
中科院分区：
文献类型：
--
作者：
Xie, Min;Ma, Tianshi;De, Wei
多项研究表明，长链非编码RNA（IncRNA）广泛参与人类癌症的恶性进展。最近报道长基因间非蛋白编码RNA 707（LINC00707）长度为3087，是促进肺腺癌细胞增殖和转移的重要癌基因，但其在胃癌（GC）中的作用仍不清楚。在本研究中，我们发现LINC00707在GC组织中过度表达，并与GC患者的晚期、肿瘤体积较大、淋巴结转移和较差的预后相关。体外和体内试验表明，LINC00707 促进 GC 细胞增殖和转移。从机制上讲，LINC00707 可以与 mRNA 稳定蛋白 HuR 发生大量相互作用； “LINC00707-HuR”联盟最终与VAV3/F11R、mRNA结合并增加了它们的稳定性。总而言之，我们的研究结果证明，LINC00707 可能通过调节 mRNA 稳定性作为 GC 的癌基因，并作为 GC 诊断和预后的潜在靶点。
Multiple studies have revealed that long non-coding RNAs (IncRNAs) extensively participate in human cancer malignant progression. The long intergenic non-protein coding RNA 707 (LINC00707), 3087 by in length, was recently reported to be an essential oncogene in promoting lung adenocarcinoma cell proliferation and metastasis, However, its role in gastric cancer (GC) remains unclear. In this study, we identified that LINC00707 was excessively expressed in GC tissues and correlated with advanced stage, larger tumor size, lymph node metastasis and poorer prognosis in GC patients. In vitro and in vivo assays showed that LINC00707 promote GC cell proliferation and metastasis. Mechanistically, LINC00707 could abundantly interact with mRNA stabilizing protein HuR; "LINC00707-HuR" coalition ulteriorly combined with VAV3/F11R, mRNAs and increased their stability. Taken together, our findings prove that LINC00707 may act as an oncogene in GC by regulating mRNA stability and serve as a potential target for GC diagnosis and prognosis.