Iminocoumarin-based red to near-infrared fluorescent turn-on probe with a large Stokes shift for imaging H2S in living cells and animals
Iminocoumarin-based red to near-infrared fluorescent turn-on probe with a large Stokes shift for imaging H2S in living cells and animals
复制标题
基于亚氨基香豆素的红色至近红外荧光开启探针，具有大斯托克斯位移，用于活细胞和动物中的 H2S 成像
DOI：
10.1016/j.dyepig.2018.12.029
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发表时间：
2019-04-01
影响因子：
4.5
通讯作者：
Feng, Guoqiang
中科院分区：
文献类型：
--
作者：
Feng, Shumin;Xia, Qingfeng;Feng, Guoqiang
近年来，用于检测生物体系中硫化氢的有机染料荧光探针的发展引起了人们的极大关注。在这项工作中，我们开发了一种基于四氢喹恶啉亚胺香豆素的红色到近红外(Red-to-NIR)的荧光探针来检测硫化氢。该探针显示了一种快速、清晰的红色到近红外荧光开启检测H_2S的过程，具有高选择性和高灵敏度(在生理pH下，检测下限低至10 nm)。此外，该探针具有显著的大斯托克斯位移(128 Nm)和低细胞毒性，可用于活体细胞和动物的H_2S成像。所有这些结果表明，这种新的探针在体内和体外都有很好的检测硫化氢的应用前景。
The development of organic dye-based fluorescent probes for detection of H2S in living systems has attracted considerable attention in recent years. In this work, a novel tetrahydroquinoxaline iminocoumarin-based red to near-infrared (red-to-NIR) fluorescent probe was developed for detection of H2S. This probe shows a rapid and distinct red-to-NIR fluorescent turn-on detection process for H2S with high selectivity and sensitivity (the detection limit was determined to be as low as 10 nM at physiological pH). In addition, this probe exhibits a remarkable large Stokes shift (128 nm) and low cytotoxicity, and can be applied for imaging H2S in living cells and animals. All these results demonstrated that this new probe is promising for detection of H2S both in vitro and in vivo.