Periodontal wound healing/regeneration of two-wall intrabony defects following reconstructive surgery with cross-linked hyaluronic acid-gel with or without a collagen matrix: a preclinical study in dogs
Periodontal wound healing/regeneration of two-wall intrabony defects following reconstructive surgery with cross-linked hyaluronic acid-gel with or without a collagen matrix: a preclinical study in dogs
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DOI：
10.3290/j.qi.b937003
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发表时间：
2021-04-01
影响因子：
1.9
通讯作者：
Sculean, Anton
中科院分区：
文献类型：
--
作者：
Shirakata, Yoshinori;Imafuji, Takatomo;Sculean, Anton
目的：体外数据表明，交联透明质酸（HA）可增强参与牙周伤口愈合/再生的细胞的增殖和迁移特性，稳定血凝块，减少炎症反应，并促进血管生成。本研究的目的是从组织学上评估单独使用交联HA或与胶原基质（CM）结合使用对骨内缺损牙周伤口愈合/再生的影响。方法和材料：在6只比格犬的下颌前磨牙的远中侧，通过手术造成双壁骨内缺损（5 mm宽，5 mm深）。24个缺损随机治疗如下：开放式皮瓣清创术（OFD）+ HA、OFD + CM、OFD + HA + CM（HA/CM）和单独OFD（对照）。在2个月时，对动物进行安乐死以进行组织学评价。结果：HA（2.43 +/- 1.25 mm）和HA/CM（2.60 +/- 0.99 mm）组与OFD（0.55 +/- 0.99 mm）组相比，在统计学上显著（P <0.05）产生了更大的新附着形成（即，与新形成的骨相邻的新牙骨质的线性长度，插入胶原纤维）。在四个处理组中，HA/CM组显示出最高量的再生组织，尽管在HA和HA/CM组之间未观察到任何组织测量参数的统计学显著差异。结论：在他们的限度内，可以得出结论，交联HA单独或与CM组合，促进牙周伤口愈合/再生的双壁骨内缺损的狗。
Objectives: In-vitro data have shown that cross-linked hyaluronic acid (HA) enhances the proliferative and migratory properties of cells involved in periodontal wound healing/regeneration, stabilizes the blood clot, reduces the inflammatory response, and facilitates angiogenesis. The aim of this study was to histologically evaluate the effects of cross-linked HA alone or combined with a collagen matrix (CM) on the periodontal wound healing/ regeneration in intrabony defects. Method and materials: Two-wall intrabony defects (5 mm wide, 5 mm deep) were surgically created at the distal and mesial aspects of mandibular premolars in six beagle dogs. The 24 defects were randomly treated as follows: open flap debridement (OFD) + HA, OFD + CM, OFD + HA + CM (HA/CM), and OFD alone (control). At 2 months, the animals were eutha-nized for histologic evaluation. Results:The HA (2.43 +/- 1.25 mm) and HA/CM (2.60 +/- 0.99 mm) groups yielded statistically significantly (P < .05) greater formation of new attachment (ie, linear length of new cementum adjacent to newly formed bone, with inserting collagen fibers) compared with the OFD (0.55 +/- 0.99 mm) group. Among the four treatment groups, the HA/CM group demonstrated the highest amount of regenerated tissues, although no statistically significant differences in any of the histometric parameters were observed between the HA and HA/CM groups. Conclusion: Within their limits, it can be concluded that cross-linked HA alone or combined with CM promotes periodontal wound healing/regeneration in two-wall intrabony defects in dogs.