Egfl7, a novel epidermal growth factor-domain gene expressed in endothelial cells
Egfl7, a novel epidermal growth factor-domain gene expressed in endothelial cells
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DOI：
10.1002/dvdy.20063
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发表时间：
2004-06-01
影响因子：
2.5
通讯作者：
Stuhlmann, H
中科院分区：
文献类型：
--
作者：
Fitch, MJ;Campagnolo, L;Stuhlmann, H
我们报道了一种新的表皮生长因子（EGF）结构域基因的克隆和表征，该基因是在逆转录病毒基因包裹筛选中发现的，并在内皮细胞中表达。该基因编码278个氨基酸的蛋白，具有一个氨基末端信号肽和两个位于中心的egf样结构域。我们根据小鼠基因组信息学小组的指导方针将这个新基因命名为Egfl7，即egf样结构域7。Egfl7 mRNA在高度血管化的成人组织中表达，如肺、心脏、子宫和卵巢。此外，Egf/7在小鼠胚胎发生早期和未分化的小鼠胚胎干细胞中表达。通过原位杂交分析胚胎9.5天胚中Egfl7的表达，发现Egf/7在胚体和卵黄囊的维管结构以及成血管细胞中胚层前体部位均有表达。在细胞内，EGFL7蛋白定位于内质网和高尔基体，表明该蛋白是分泌的靶点。事实上，重组EGFL7在瞬时转染的HEK293细胞的上清培养基中很容易检测到。我们还报道了Egfl7的一个类似物Egfl8的鉴定，并表明Egfl8蛋白与Egfl7具有相似的结构域和分子量。(C) 2004 Wiley-Liss, Inc。
We report the cloning and characterization of a novel epidermal growth factor (EGF) domain gene that was identified in a retroviral gene entrapment screen and is expressed in endothelial cells. This gene encodes a protein of 278 amino acids with an amino-terminal signal peptide and two centrally located EGF-like domains. We have named this novel gene in accordance with the guidelines of the Mouse Genome Informatics group Egfl7, for EGF-like domain 7. Egfl7 mRNA is expressed in highly vascularized adult tissues such as the lung, heart, uterus, and ovary. In addition, Egf/7 is expressed early during mouse embryogenesis and in undifferentiated murine embryonic stem cells. The analysis of Egfl7 expression in embryonic day 9.5 embryos by in situ hybridization indicates that Egf/7 is expressed in vascular structures in both the embryo proper and the yolk sac and at sites of mesodermal precursors of angioblasts. Within the cell, EGFL7 protein is localized to the endoplasmic reticulum and Golgi apparatus, suggesting that the protein is targeted for secretion. Indeed, recombinant EGFL7 is readily detectable in the supernatant media of transiently transfected HEK293 cells. We also report the identification of an Egfl7 paralog, Egfl8, and show that EGFL8 protein shares similar domains and molecular weight with EGFL7. (C) 2004 Wiley-Liss, Inc.