Increase in immunogenicity with concomitant loss of tumorigenicity of respiratory tract carcinomas during in vitro culture.
Increase in immunogenicity with concomitant loss of tumorigenicity of respiratory tract carcinomas during in vitro culture.
复制标题
在体外培养过程中，呼吸道癌的免疫原性增加，同时致瘤性丧失。
作者：
R. Jamasbi;P. Nettesheim
细胞系建立在体外由致癌性，多环烃诱导大鼠呼吸道癌。癌细胞系在体外的增殖导致致瘤性的进行性降低。在X射线照射，免疫抑制受体和免疫重建受体的肿瘤移植研究表明，细胞被拒绝，因为它们的免疫原性，因为高发病率的肿瘤被观察到在X射线照射受体，但不是在正常或X射线照射，重建受体。当免疫功能正常的大鼠用来自体外肿瘤系的细胞免疫时，产生了强烈的肿瘤移植抗性。类似的免疫接种与相应的体内肿瘤细胞系引起非常少的保护，如果有的话，和免疫接种与非交叉反应的肉瘤细胞系在体外生长没有产生针对癌细胞系的免疫保护。在免疫抑制受体中体外适应的肿瘤系的单次体内传代完全恢复了致瘤性。体外培养的癌的免疫原性增加似乎涉及癌固有的预先存在的血管生成素，而不是在组织培养过程中获得的新抗原，例如与逆转录病毒、支原体或异源血清相关的抗原。
Cell lines were established in vitro from respiratory tract carcinomas induced in rats by carcinogenic, polycyclic hydrocarbons. Propagation of the carcinoma lines in vitro lead to a progressive decrease in tumorigenicity. Tumor transplantation studies in X-irradiated, immunosuppressed recipients and in immunologically reconstituted recipients suggested that the cells are rejected because of their immunogenicity, since a high incidence of tumors was observed in X-irradiated recipients but not in normal or X-irradiated, reconstituted recipients. When immunologically competent rats were immunized with cells from an in vitro tumor line, strong tumor transplantation resistance resulted. Similar immunization with the corresponding in vivo tumor line caused very little if any protection, and immunization with a non-cross-reacting sarcoma line grown in vitro did not produce immunological protection against carcinoma cell lines. A single in vivo passage of the in vitro-adapted tumor line in immunosuppressed recipients fully restored tumorigenicity. The increase in immunogenicity of carcinomas cultured in vitro appears to involve preexisting angigens indigenous to the carcinomas rather than new antigens acquired during tissue culture, such as antigens related to retroviruses, mycoplasmas, or heterologous serum.