OPTICAL ANALYSIS OF SYNAPTIC VESICLE RECYCLING AT THE FROG NEUROMUSCULAR-JUNCTION
OPTICAL ANALYSIS OF SYNAPTIC VESICLE RECYCLING AT THE FROG NEUROMUSCULAR-JUNCTION
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DOI：
10.1126/science.1553547
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发表时间：
1992-01-10
期刊：
影响因子：
56.9
通讯作者：
BEWICK, GS
中科院分区：
文献类型：
--
作者：
BETZ, WJ;BEWICK, GS
荧光染料FM 1 -43和RH 414以活性依赖的方式标记运动神经末梢，所述活性依赖的方式涉及被回收的突触囊泡的染料摄取。这允许光学监测活神经末梢中的囊泡回收，以确定回收的囊泡如何重新进入囊泡池。结果表明，回收囊泡混合池形态和功能。一个完整的周期释放的发射，回收囊泡，并重新释放的发射似乎需要约1分钟。
The fluorescent dyes FM1-43 and RH414 label motor nerve terminals in an activity-dependent fashion that involves dye uptake by synaptic vesicles that are recycling. This allows optical monitoring of vesicle recycling in living nerve terminals to determine how recycled vesicles reenter the vesicle pool. The results suggest that recycled vesicles mix with the pool morphologically and functionally. One complete cycle of release of transmitter, recycling of a vesicle, and rerelease of transmitter appears to take about 1 minute.