Albumin enhanced morphometric image analysis in CLL
Albumin enhanced morphometric image analysis in CLL
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DOI：
10.1002/cyto.b.10059
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发表时间：
2004-01-01
影响因子：
3.4
通讯作者：
Marti, GE
中科院分区：
文献类型：
--
作者：
Lunning, MA;Zenger, VE;Marti, GE
背景资料：从慢性淋巴细胞白血病（CLL）和血膜伪影患者的淋巴细胞的异质性，使形态学亚分类，这种疾病的difficult.Methods：我们回顾了配对的血膜制备乙二胺四乙酸（ETDA）样品与牛血清白蛋白（BSA）从82例CLL患者。第1组遵守NCCLS关于EDTA血膜制备的质量标准。第2组由含有EDTA和1：12稀释的22%BSA的血膜组成。选取8例患者进行数字显微镜检查和统计学分析。结果：第1组的平均细胞面积+/-标准误差为127.8 μ M（2）+/- 1.42（n = 793），而第2组为100.7 μ M（2）+/- 1.39（n = 831）。第1组的细胞核面积为88.9 μ M（2）+/- 0.85，而第2组为76.4 μ M（2）0.83。对于细胞核透射率，第1组的值为97.6 +/- 0.85，第2组为104.1 +/- 0.83。组1的细胞核：细胞质比为0.71 +/- 0.003，组2为0.78 +/- 0.003。结论：添加BSA可使非典型淋巴细胞减少，污迹细胞减少。BSA还降低淋巴细胞面积和核面积，而核透射率和核质比增加。一个标准化的玻片制备方法将允许准确的实验室间比较。BSA的使用可能允许更好地实施基于血膜的CLL亚分类，并导致形态学与细胞遗传学和免疫表型更好的相关性。2003年出版Wiley-Liss，Inc（匕首）。
Background: The heterogeneity of lymphocytes from patients with chronic lymphocytic leukemia (CLL) and blood film artifacts make morphologic subclassification of this disease difficult.Methods: We reviewed paired blood films prepared from ethylene-diamine-tetraacetic acid (ETDA) samples with and without bovine serum albumin (BSA) from 82 CLL patients. Group 1 adhered to NCCLS specifications for the preparations of EDTA blood films. Group 2 consisted of blood films containing EDTA and a 1:12 dilution of 22% BSA. Eight patients were selected for digital photomicroscopy and statistical analysis. Approximately 100 lymphocytes from each slide were digitally captured.Results: The mean cell area +/- standard error was 127.8 muM(2) +/- 1.42 for (n = 793) for group 1 versus 100.7 muM(2) +/- 1.39 (n = 831) for group 2. The nuclear area was 88.9 muM(2) +/- 0.85 for group 1 versus 76.4 muM(2) 0.83 for group 2. For the nuclear transmittance, the values were 97.6 +/- 0.85 for group 1 and 104.1 +/- 0.83 for group 2. The nuclear:cytoplasmic ratios were 0.71 +/- 0.003 for group 1 and 0.78 +/- 0.003 for group 2. All differences were statistically significant (P < 0.001).Conclusions: BSA addition results in the reduction of atypical lymphocytes and a decrease in smudge cells. BSA also decreases the lymphocyte area and nuclear area, whereas nuclear transmittance and nuclear:cytoplasmic ratio are increased. A standardized method of slide preparation would allow accurate interlaboratory comparison. The use of BSA may permit better implementation of the blood film-based subclassification of CLL and lead to a better correlation of morphology with cytogenetics and immunophenotyping. Published 2003 Wiley-Liss, Inc(dagger).