Genome-wide analysis of gene expression reveals function of the bZIP transcription factor HY5 in the UV-B response of Arabidopsis
Genome-wide analysis of gene expression reveals function of the bZIP transcription factor HY5 in the UV-B response of Arabidopsis
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DOI：
10.1073/pnas.0308044100
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发表时间：
2004-02-03
影响因子：
11.1
通讯作者：
Nagy, F
中科院分区：
文献类型：
--
作者：
Ulm, R;Baumann, A;Nagy, F
光环境是植物整个生命周期中控制许多发育过程的关键因素。一个重要的和不断增加的部分入射阳光包括UV-B区域（280-320 nm）的一部分，它不完全被地球平流层中的臭氧层吸收。这部分不可避免地到达无柄植物的太阳辐射，既可以作为环境胁迫因子，也可以作为信息信号。为了鉴定参与UV响应的拟南芥基因，我们通过使用包含几乎完整的拟南芥基因组（> 24，000个基因）的高密度寡核苷酸微阵列来监测UV-B照射的幼苗的基因表达谱。一个强大的早期低水平UV-B响应基因，100激活和7抑制，被确定。在所有的情况下分析，UV-B诱导被认为是独立的已知的光感受器。这组基因被认为代表了由难以捉摸的UV-B光感受器触发的信号级联的分子读出。此外，我们的分析确定了细胞对不同UV-B范围的反应之间的相互作用，这使我们假设存在部分不同但相互作用的UV-B感知和信号传导机制。最后，我们证明了bZIP转录因子HY 5是UV-B介导的调节基因的子集所必需的。
The light environment is a key factor that governs a multitude of developmental processes during the entire life cycle of plants. An important and increasing part of the incident sunlight encompasses a segment of the UV-B region (280-320 nm) that is not entirely absorbed by the ozone layer in the stratosphere of the earth. This portion of the solar radiation, which inevitably reaches the sessile plants, can act both as an environmental stress factor and an informational signal. To identify Arabidopsis genes involved in the UV response, we monitored the gene expression profile of UV-B-irradiated seedlings by using high-density oligo-nucleotide microarrays comprising almost the full Arabidopsis genome (>24,000 genes). A robust set of early low-level UV-B-responsive genes, 100 activated and 7 repressed, was identified. In all cases analyzed, UV-B induction was found to be independent of known photoreceptors. This group of genes is suggested to represent the molecular readout of the signaling cascade triggered by the elusive UV-B photoreceptor(s). Moreover, our analysis identified interactions between cellular responses to different UV-B ranges that led us to postulate the presence of partially distinct but interacting UV-B perception and signaling mechanisms. Finally, we demonstrate that the bZIP transcription factor HY5 is required for UV-B-mediated regulation of a subset of genes.