Association of the plasma and tissue riboflavin levels with C20orf54 expression in cervical lesions and its relationship to HPV16 infection.
Association of the plasma and tissue riboflavin levels with C20orf54 expression in cervical lesions and its relationship to HPV16 infection.
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宫颈病变中血浆和组织核黄素水平与C20orf54表达的关联及其与HPV16感染的关系
DOI：
10.1371/journal.pone.0079937
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Upur H
中科院分区：
文献类型：
--
作者：
Aili A;Hasim A;Kelimu A;Guo X;Mamtimin B;Abudula A;Upur H
核黄素缺乏会引起各种代谢问题，导致皮肤和粘膜紊乱。有限的证据表明，大量摄入核黄素可能会降低患癌症的总体风险。然而，这种缺乏与宫颈癌和癌前病变的关系仍不明确。在本研究中，我们描述了宫颈上皮内瘤变（CIN）和宫颈鳞状细胞癌（CSCC）患者血浆和组织核黄素水平与C20orf54蛋白表达的关系，以及这些水平与人乳头瘤病毒16,18 （HPV16/18）感染的关系。采用高效液相色谱法（HPLC）检测CIN和CSCC患者血液核黄素水平，采用酶联免疫吸附试验（ELISA）检测CSCC患者及匹配的正常粘膜上皮组织核黄素水平。采用qRT-PCR和western blot分别检测C20orf54在新鲜CSCC和配对组织中的表达。并通过免疫组化（IHC）方法对其进行验证，分别采用福尔马林固定、石蜡包埋CIN和CSCC。采用HPV基因分型芯片对HPV感染进行分析和分型。结果显示，与正常对照相比，CIN和CSCC患者血浆核黄素水平降低。与正常宫颈上皮细胞相比，CSCC患者组织中的核黄素也显著降低。C20orf54在CSCC中的mRNA和蛋白水平均显著上调。与HPV16/18阴性组织相比，HPV16/18阳性组织中组织核黄素水平显著降低，组织核黄素水平与C20orf54 mRNA和蛋白表达呈负相关。此外，C20orf54与肿瘤分期有显著相关性。综上所述，C20orf54可能通过调节核黄素的吸收在维吾尔族宫颈癌发生过程中发挥保护作用，并与HPV感染有关。
Riboflavin deficiency can cause a variety of metabolic problems that lead to skin and mucosal disorders. Limited evidence suggests that high intake of riboflavin may reduce overall risks of cancer. However, association of this deficiency with cervical cancer and precancerous lesions are still not definitively known. In this study, we characterized the relationship between plasma and tissue riboflavin levels and C20orf54 protein expression in patients with cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma (CSCC) as well as the relationship of these levels with human papillomavirus virus 16, 18 (HPV16/18) infections. High-performance liquid chromatography (HPLC) was used to measure blood riboflavin levels in patients with CIN and CSCC, and an enzyme-linked immunosorbent assay (ELISA) was used to determine tissue riboflavin levels in patients with CSCC and matched normal mucous epithelia. The expression of C20orf54 in fresh CSCC and matched tissues were detected by qRT-PCR and western blot, respectively. And it was further confirmed by immunohistochemistry (IHC) with formalin-fixed, paraffin-embedded CIN and CSCC. An HPV genotyping chip was used to analyze HPV infection and typing. The results showed that patients with CIN and CSCC had decreased plasma riboflavin levels as compared with normal controls. There was also significantly decreased riboflavin in tissues from CSCC patients, when compared with normal cervical epithelia. C20orf54 expression were significantly up-regulated in CSCC compared to matched control on both mRNA and protein level. Tissue riboflavin levels were significantly lower in HPV16/18 positive tissue compared with HPV16/18-negative tissue, and an inverse association was found between tissue riboflavin levels and C20orf54 mRNA and protein expression in CSCC. Additionally, C20orf54 was significantly correlated with tumor stages. In conclusion, C20orf54 tend to play a protective role in Uyghur cervical carcinogenesis of which modulating riboflavin absorption, and it is also related with HPV infection.
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影响因子：
4.2
作者：
Powers, HJ
通讯作者：
Powers, HJ
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作者：
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