First report of OXA-48-producing Klebsiella pneumoniae strains in Iran
First report of OXA-48-producing Klebsiella pneumoniae strains in Iran
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DOI：
10.3205/dgkh000227
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发表时间：
2014-01-01
影响因子：
3
通讯作者：
Bonnin, Remy A.
中科院分区：
文献类型：
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作者：
Azimi, Leila;Nordmann, Patrice;Bonnin, Remy A.
碳青霉烯类耐药肠杆菌科细菌在世界范围内的报道越来越多，并在卫生保健机构造成治疗问题。本研究采用表型和基因分型方法检测了28株耐亚胺培南肺炎克雷伯菌碳青霉烯酶的表达。采用改良Hodge试验(MHT)、CarbaNP试验进行表型检测，并用特异性引物对BLA(OXA-48)-、BLA(KPC)-、BLA(NDM)-和BLA(VIM)型碳青霉烯酶进行聚合酶链式反应。所有耐亚胺培南肺炎克雷伯菌MHT和CarbaNP试验均为阳性。在28株分离株中有27株检测到blA(OXA-48)基因。1株BLA(vim-4)基因阳性。根据我们的结果，NP试验和MHT试验对检测这些碳青霉烯酶具有较高的敏感性和特异性。这项研究报告了伊朗第一例产生OXA-48肺炎克雷伯菌的病例。
Carbapenem-resistant Enterobacteriaceae are increasingly reported worldwide and cause therapeutic problem in health care facilities. In this study 28 imipenem-resistant K. pneumoniae were examined for expression of carbapenemases by phenotypic and genotypic methods. Modified Hodge Test (MHT), CarbaNP test were used for phenotypic detection, and PCR using specific primers for the detection of bla(OXA-48)-, bla(KPC)-, bla(NDM)- and bla(VIM) -type carbapenemases with specific primers were performed. MHT and CarbaNP tests were positive for all of imipenem-resistant K. pneumoniae. The bla(OXA-48) gene was detected in 27/28 isolates. One isolate was positive for the presence of the bla(VIM-4) gene. According to our results NP test and MHT have high sensitivity and specificity for detection of those carbapenemases. This study reports the first cases of OXA-48-producing K. pneumoniae in Iran.