Characterization of microtiterplates with integrated optical sensors for oxygen and pH, and their applications to enzyme activity screening, respirometry, and toxicological assays
Characterization of microtiterplates with integrated optical sensors for oxygen and pH, and their applications to enzyme activity screening, respirometry, and toxicological assays
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DOI：
10.1016/j.snb.2005.07.056
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发表时间：
2006-02-27
影响因子：
8.4
通讯作者：
Klimant, I
中科院分区：
文献类型：
--
作者：
Arain, S;John, GT;Klimant, I
本文介绍了带有集成荧光 pH 和氧气传感器的微孔板 (MTP) 的特性，及其在酶筛选和细菌呼吸活动监测中的应用。由分析物敏感指示剂和参考荧光团组成的薄亲水传感膜沉积在 MTP 底部，这允许对 pH 和 pO(2) 进行免校准定量，并具有可接受的精度和分辨率，适用于酶活性筛查、呼吸测量或毒理学测定等应用。主要研究传感器特性是否适合此类应用。具体而言，使用氧敏感板对葡萄糖氧化酶进行酶活性筛选，并对大肠杆菌和恶臭假单胞菌进行细菌生长监测。此外，监测恶臭假单胞菌呼吸活性的毒理学测定被转换为微孔板形式。 (c) 2005 Elsevier B.V. 保留所有权利。
This paper describes properties of microplates (MTPs) with integrated, fluorescence-based sensors for pH and oxygen, and their application for enzyme screening and monitoring of bacterial respiratory activity. Thin, hydrophilic sensing films consisting of an analyte-sensitive indicator and a reference fluorophore, are deposited on the bottom of the MTP This allows for calibration-free quantification of pH and pO(2) with an acceptable accuracy and resolution for applications such as enzyme activity screening, respirometry, or toxicological assays. The sensor properties are mainly investigated with respect to their qualification for such applications. Specifically, enzyme activity screening is demonstrated for glucose oxidase using oxygen-sensitive plates, and for bacterial growth monitoring of Escherichia coli and Pseudomonas putida. Furthermore, a toxicological assay monitoring the respiration activity of P. putida was converted into a microplate format. (c) 2005 Elsevier B.V. All rights reserved.