SNIP1: a new activator of HSE signaling pathway
SNIP1: a new activator of HSE signaling pathway
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DOI：
10.1007/s11010-011-1120-y
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发表时间：
2012-03-01
影响因子：
4.3
通讯作者：
Yu, Long
中科院分区：
文献类型：
--
作者：
Li, Qiang;An, Jian;Yu, Long
SNIP 1（Smad nuclear interacting protein 1，Smad核相互作用蛋白1）作为一种转录辅激活因子，近10年来受到越来越多的关注。我们在这里报告，通过定量实时PCR分析在18个不同的人体组织中，SNIP 1被发现普遍表达。当在HeLa细胞中过表达时，SNIP 1-EGFP融合蛋白表现出核定位，在某些细胞中具有特征性的亚核斑点状分布或形成较大的离散核体。报告基因分析显示SNIP 1在HEK 293细胞和H1299细胞中的过表达强烈激活HSE信号通路。此外，SNIP 1还可以选择性地调节HSP 70 A1 A和HSP 27的转录。综上所述，我们的研究结果表明SNIP 1也可能是HSE信号通路的正调控因子。
In the last 10 years, more and more attention has been focused on SNIP1 (Smad nuclear interacting protein 1), which functions as a transcriptional coactivator. We report here that through quantitative real-time PCR analysis in 18 different human tissues, SNIP1 was found to be expressed ubiquitously. When overexpressed in HeLa cells, SNIP1-EGFP fused protein exhibited a nuclear localization with a characteristic subnuclear distribution in speckles or formed larger discrete nuclear bodies in some cells. Reporter gene assay showed that overexpression of SNIP1 in HEK 293 cells or H1299 cells strongly activated the HSE signaling pathway. Moreover, SNIP1 could selectively regulate the transcription of HSP70A1A and HSP27. Taken together, our findings suggest that SNIP1 might also be a positive regulator of HSE signaling pathway.