FINE STRUCTURE OF ERYTHROCYTIC STAGES OF 3 AVIAN MALARIAL PARASITES PLASMODIUM FALLAX P LOPHURAE AND P CATHEMERIUM
FINE STRUCTURE OF ERYTHROCYTIC STAGES OF 3 AVIAN MALARIAL PARASITES PLASMODIUM FALLAX P LOPHURAE AND P CATHEMERIUM
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DOI：
10.4269/ajtmh.1966.15.449
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发表时间：
1966-01-01
影响因子：
3.3
通讯作者：
AIKAWA, M
中科院分区：
文献类型：
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作者：
AIKAWA, M
本文研究了禽疟原虫（Plasmodium fallax、P. lophurae和P. cathemerium）红细胞期的细微结构，特别强调了它们的形态进化。将从显示40-60%寄生虫血症的鸟获得的血液固定在戊二醛中，后固定在OSO 4中，并包埋在epon中。在RCA EMU-3G电子显微镜中检查用醋酸双氧铀和柠檬酸铅复染的薄切片。裂殖子结构复杂，包括锥状体、成对细胞器、致密体、球状体、胞口、膜复合体、核、胞囊、内质网和核糖体等。当裂殖子感染新的宿主细胞时，它会聚集并失去大部分复杂的结构，只保留生长所需的重要细胞器，如细胞核、胞囊、细胞口、内质网和核糖体。随着寄生虫的生长，裂殖子通过分裂的方式增加数量，并且在裂殖子阶段不活动的细胞口开始摄取宿主细胞的细胞质。通过一系列连续的核分裂，一个单核滋养体长成一个单核体。细胞质分裂后，一旦双质体已达到其完整的大小。首先，厚内膜的片段随机地重新出现，新形成的裂殖子开始从这些区域突出。当裂殖子从中央细胞质团中出芽时，感染时裂殖子丢失的结构戏剧性地重新出现。通过这个过程，大约12个新的裂殖子由寄生虫形成。
The fine structure of the erythrocytic stages of avian malarial parasites, Plasmodium fallax, P. lophurae, and P. cathemerium were studied with particular emphasis on their morphological evolution. Blood obtained from birds showing 40-60% parasitemia was fixed in glutaraldehyde, postfixed in OSO4, and embedded in epon. Thin sections, counter-stained with uranyl acetate and lead citrate, were examined in an RCA EMU-3G electron microscope. Merozoites of these avian malarial parasites showed a highly complex structure including such organelles as a conoid, paired organelles, dense bodies, spherical body, cytostome, pellicular complex, nucleus, mitochondrion, endoplasmic reticulum and ribo-somes. When a merozoite infects a new host cell it rounds up and loses much of its complex structure, retaining only the vital organelles for growth such as a nucleus, mitochondrion, cytostome, endoplasmic reticulum and ribosomes. As the parasite grows, the mitochondrion increases in number by means of fission and the cytostome, inactive during the merozoite stage, starts to ingest host cell cytoplasm. Through a series of successive nuclear divisions, a uninuclear trophozoite grows into a schizont. Cytoplasmic segmentation follows once the schizont has reached its full size. First the segments of the thick inner membrane of the pellicle reappears at random and from these areas the newly forming merozoites start to protrude. As the merozoites bud off from the central cytoplasmic mass the structures which were lost by the merozoite upon infection dramatically reappear. Through this process approximately 12 new merozoites are formed from a parasite.