Semi-micro, dye-binding assay for rabbit interferon.
Semi-micro, dye-binding assay for rabbit interferon.
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兔干扰素的半微量染料结合测定。
DOI：
10.1128/aem.21.4.723-725.1971
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发表时间：
1971
期刊：
影响因子：
--
通讯作者：
J. A. Armstrong
中科院分区：
文献类型：
--
作者：
J. A. Armstrong
干扰素测定的半微量方法基于6 mm威尔斯孔中细胞病变效应抑制的定量。将培养物暴露于干扰素稀释液，用水泡性口炎病毒攻击，并用氯化甲基玫瑰碱染色。然后洗脱结合的染料并进行比色测量。该方法比空斑抑制法更灵敏，且非常经济。培养年限是影响繁殖力的最重要因素。
The semi-micro method for interferon assay was based on quantitation of inhibition of cytopathic effects in 6-mm wells. Cultures were exposed to interferon dilutions, challenged with vesicular stomatitis virus, and stained with methylrosanaline chloride. The bound dye was then eluted and measured colorimetrically. The method was more sensitive than the plaque-inhibition method and was extremely economical. Culture age was the most important factor affecting reproductibility.