Functional and morphologic characterization of human T cells continuously grown in vitro.
Functional and morphologic characterization of human T cells continuously grown in vitro.
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DOI：
10.4049/jimmunol.119.1.131
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发表时间：
1977-07
影响因子：
4.4
通讯作者：
F. Ruscetti;D. Morgan;R. Gallo
中科院分区：
文献类型：
--
作者：
F. Ruscetti;D. Morgan;R. Gallo
通过使用从有丝分裂原刺激的人外周血淋巴细胞中获得的培养基中存在的一种因子，从许多正常的人骨髓和外周血样本中获得胸腺源淋巴细胞的长期生长（现在超过13个月）。这种长期生长既不能单独由有丝分裂原启动，也不能单独维持。所有细胞培养均大于90%呈E型玫瑰花结阳性，而B细胞标记物、表面IgG和IgM、EAC玫瑰花结常规阴性。没有证据表明在这些培养物中存在粒细胞、单核细胞及其前体。然后对E型玫瑰花结阳性细胞进行测试，看它们是否具有T细胞功能。PHA， Con A和美洲商陆有丝分裂原在这些培养物中刺激淋巴增殖反应，与新鲜外周血细胞的反应相当。这些增殖的细胞也能够释放细胞介质，如干扰素和集落刺激活性。这些培养细胞的T淋巴细胞性质的进一步证据是从单向混合白细胞培养中获得的，这些细胞对同种异体细胞有反应，但不能刺激。这些细胞的功能和形态特征表明，这些细胞是体外连续生长的T细胞。
Long-term growth (now over 13 months) of thymus-derived lymphocytes from numerous normal human bone marrow and peripheral blood cell samples was accomplished by using a factor present in media obtained from mitogen-stimulated human peripheral blood lymphocytes. This long-term growth could neither be initiated nor maintained by mitogens alone. All cell cultures were greater than 90% E rosette-positive, whereas the tests for B cell markers, surface IgG and IgM, and EAC rosette were routinely negative. There was no evidence for the presence of granulocytes, monocytes, and their precursors in these cultures. The E rosette-positive cells were then tested to see if they had T cell functions. PHA, Con A, and pokeweed mitogens stimulated lymphproliferative responses in these cultures comparable to those of fresh peripheral blood cells. These proliferating cells were also able to release cell mediators, such as interferon and colony-stimulating activity. Further evidence for the T lymphocyte nature of these cultured cells was obtained from one-way mixed leukocyte cultures in which these cells responded to but were unable to stimulate allogeneic cells. The functional and morphologic characteristics of these cultured cells show that these cells are T cells that grow continuously in vitro.