A Genome-Wide Longitudinal Transcriptome Analysis of the Aging Model Podospora anserine
A Genome-Wide Longitudinal Transcriptome Analysis of the Aging Model Podospora anserine
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DOI：
10.1371/journal.pone.0083109
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发表时间：
2013-12-20
期刊：
影响因子：
3.7
通讯作者：
Osiewacz, Heinz D.
中科院分区：
文献类型：
--
作者：
Philipp, Oliver;Hamann, Andrea;Osiewacz, Heinz D.
生物系统的老化，由对基因表达有潜在影响的各种过程控制。在这里，我们报告了对真菌衰老模型鹅座孢霉的全基因组分析。3个年龄确定的个体的总RNA被汇集在一起，并用SuperSAGE(基因表达序列分析)进行分析。生物信息学分析师确定了在衰老过程中受影响的不同分子途径。虽然与核糖体和蛋白酶体质量控制系统相关的转录本的丰度随着年龄的增长而减少，但与自噬相关的转录本的丰度增加，这表明自噬可能是一种代偿质量控制途径。与能量代谢相关的转录谱，包括线粒体功能，被发现在衰老过程中会发生波动。将在衰老过程中不断下调的野生型转录本与长期存活的铜吸收突变体中下调的转录本进行比较，验证了细胞铜代谢与年龄相关的变化的相关性。总体而言，我们(I)提供了实验性衰老模型P.anserina的纵向研究的独特的与年龄相关的数据集，这为未来在各种生物体中的研究提供了参考资源；(Ii)建议自噬是一个关键的质量控制，一旦其他途径失败，它就会变得活跃；(Iii)为后续的实验研究提供可测试的预测。
Aging of biological systems controlled by various processes which have a potential impact on gene expression. Here we report a genome-wide an scriptome analysis of the fungal aging model Podospora anserina. Total RNA of three individuals defined age were pooled and analyzed by SuperSAGE (serial analysis of gene expression). A bioinformatics analysts identified different molecular pathways to be affected during aging. While the abundance of transcripts linked to ribosomes and to the proteasome quality control system were found to decrease during aging, those associated with autophagy increase, suggesting that autophagy may act as a compensatory quality control pathway. Transcript profiles associated with the energy metabolism including mitochondrial functions were identified to fluctuate during aging. Comparison of wild type transcripts, which are continuously down-regulated during aging, with those down-regulated in the long-lived, copper-uptake mutant grisea, validated the relevance of age-related changes in cellular copper metabolism. Overall, we (i) present a unique age-related data set of a longitudinal study of the experimental aging model P. anserina which represents a reference resource for future investigations in a variety of organisms, (ii) suggest autophagy to be a key quality control hat becomes active once other pathways fail, and (iii) present testable predictions for subsequent experimental investigations.