Silicone-grease-based immobilisation method for the preparation of enzyme electrodes.
Silicone-grease-based immobilisation method for the preparation of enzyme electrodes.
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用于制备酶电极的硅脂固定化方法。
DOI：
10.1039/an9891401427
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Mannino,S
中科院分区：
文献类型：
--
作者：
Connor,MP;Sanchez,J;Wang,J;Smyth,MR;Mannino,S
描述了一种构建电流生物传感器的方法，该方法基于在硅脂中加入酶并使用该油脂填充石墨表面上的微孔。以酪氨酸酶为例的酶-油脂电极概念为酶电极的制造提供了一种非常简单、快速且廉价的方法。酪氨酸酶电极对酚类化合物浓度的动态变化响应非常迅速。已确定响应时间 (t95%) 低至 5 秒。通过流动注射，每小时可处理 120 个样品，相对标准偏差为 2.4%。电极保持活性约 12 d。多巴胺的检测限为 6 × 10–6M。这种生物传感器构建方法应该适用于其他酶底物系统。
An approach to the construction of amperometric biosensors based on the incorporation of an enzyme in silicone grease and using the grease to fill micropores on a graphite surface is described. The enzyme-grease electrode concept, illustrated with the enzyme tyrosinase, offers a very simple, rapid and inexpensive approach to the fabrication of enzyme electrodes. The tyrosinase electrode responds very rapidly to dynamic changes in the concentration of phenolic compounds. A response time (t95%) as low as 5 s has been determined. With flow injection, 120 samples per hour can be processed with a relative standard deviation of 2.4%. The electrode remains active for about 12 d. The detection limit for dopamine is 6 × 10–6M. This method of biosensor construction should be applicable to other enzyme-substrate systems.