The interaction of Chlamydia trachomatis with host cells: ultrastructural studies of the mechanism of release of a biovar II strain from HeLa 229 cells.
The interaction of Chlamydia trachomatis with host cells: ultrastructural studies of the mechanism of release of a biovar II strain from HeLa 229 cells.
复制标题
沙眼衣原体与宿主细胞的相互作用：HeLa 229 细胞释放 Biovar II 菌株机制的超微结构研究。
DOI：
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发表时间：
1985
影响因子：
6.4
通讯作者：
H. Caldwell
中科院分区：
文献类型：
--
作者：
William J. Todd;H. Caldwell
沙眼衣原体的血清型被分类为生物型II菌株（免疫型A、Ba和B-K），目前被认为是产生以相当复杂的发病机制为特征的疾病的重要人类病原体。研究了C.沙眼衣原体（菌株UW 3/Cx，血清型D）与HeLa 229细胞的接触，以确定这些专性细胞内寄生虫的释放机制。未固定的HeLa细胞感染衣原体后的抗体染色结果表明，该生物型C。沙眼衣原体可以离开细胞而不伴随宿主细胞的死亡。通过扫描和透射电子显微镜研究了衣原体从细胞中释放的机制。超微结构观察表明，衣原体包涵体从宿主细胞质中分离，并通过类似于胞吐的过程运输到宿主细胞表面。这些观察结果之间的相互作用C。沙眼衣原体和宿主细胞在体外的相互作用可能与理解这些生物体在体内产生的复杂发病机制有关，特别是它们产生无症状或潜伏感染的能力。
Serotypes of Chlamydia trachomatis classified as biovar II strains (immunotypes A, Ba, and B-K) are currently recognized as important human pathogens that produce disease characterized by a rather complex pathogenesis. We have studied some morphological phenomena in the interaction of C. trachomatis (strain UW3/Cx, serotype D) with HeLa 229 cells to define the mechanisms of release of these obligate intracellular parasites. Fluorescent-antibody staining of unfixed HeLa cells infected with chlamydiae suggested that this biotype of C. trachomatis can exit cells without concomitant death of the host cell. The mechanisms by which chlamydiae were released from cells were studied by scanning and transmission electron microscopy. Ultrastructural observations indicated that the chlamydial inclusion was segregated from host cytoplasm and transported to the host cell surface by a process similar to exocytosis. These observations of interactions between C. trachomatis and the host cell in vitro may be relevant for understanding the complex pathogenesis these organisms produce in vivo, specifically their ability to produce asymptomatic or latent infections.
对到计划生育诊所就诊的妇女进行衣原体感染筛查。
DOI：
--
发表时间：
1983
期刊：
The Western journal of medicine
影响因子：
--
作者：
Schachter,J;Stoner,E;Moncada,J
通讯作者：
Moncada,J