Layer-specific production of nitric oxide during cortical circuit formation in postnatal mouse brain
Layer-specific production of nitric oxide during cortical circuit formation in postnatal mouse brain
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DOI：
10.1093/cercor/bhh135
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发表时间：
2005-03-01
期刊：
影响因子：
3.7
通讯作者：
Hisatsune, T
中科院分区：
文献类型：
--
作者：
Imura, T;Kanatani, S;Hisatsune, T
在发育中的大脑皮层，神经元型一氧化氮合酶（nNOS）表达丰富，但暂时。在出生后的早期阶段，位于皮层板的多层结构中的皮层神经元开始形成组织良好的皮层回路，但对层特异性回路形成的分子机制知之甚少。为了解决一氧化氮（NO）的参与，我们利用了一种新的NO指示剂（DAR-4 M），并开发了一个协议，用于实时成像的NO在新鲜的皮质切片后，N-甲基-D-天冬氨酸刺激。在出生后第0天（P0），NO的生产被限制在深层（层V和VI）的躯体感觉皮层的瞬时突触形成。在P10，NO的产生扩大到IV层，在那里大量的丘脑皮质轴突形成突触。NO的产生模式可能对应于突触形成的活性位点。这项研究是第一个明确的证明NO生产在出生后的小鼠新皮层。提出的研究结果可能反映了一个功能的NO在新皮层的神经回路的层特定的发展。
In the developing cerebral cortex, neuronal nitric oxide synthase (nNOS) is expressed abundantly, but temporarily. During the early postnatal stage, cortical neurons located in the multi-layered structure of the cortical plate start forming well-organized cortical circuits, but little is known about the molecular machinery for layer-specific circuit formation. To address the involvement of nitric oxide (NO), we utilized a new NO indicator (DAR-4M) and developed a protocol for the real-time imaging of NO produced in fresh cortical slices upon N-methyl-D-aspartic acid stimulation. At postnatal day 0 (P0), NO production was restricted to the deep layers (layers V and VI) of the somatosensory cortex where transient synapses are formed. At P10, the production of NO was expanded to layer IV where large numbers of thalamocortical axons form synapses. The pattern of NO production could correspond to active sites for synaptic formation. This study is the first clear demonstration of NO production in the postnatal mouse neocortex. The findings presented may reflect a function of NO in relation to the layer-specific development of neural circuits in the neocortex.