Distinct characteristics of resistance to Borrelia burgdorferi induced arthritis in C57BL/6N mice
Distinct characteristics of resistance to Borrelia burgdorferi induced arthritis in C57BL/6N mice
复制标题
DOI：
10.1128/iai.66.1.161-168.1998
复制
发表时间：
1998-01-01
影响因子：
3.1
通讯作者：
Weis, JJ
中科院分区：
文献类型：
--
作者：
Ma, Y;Seiler, KP;Weis, JJ
对感染伯氏疏螺旋体的小鼠的研究表明，关节炎的严重程度受宿主遗传组成的影响：C3 H小鼠发展为严重的关节炎，而BALB/c和C57 BL/6小鼠发展为轻度关节炎。在本研究中，在这三种小鼠品系中确定了增加感染剂量对关节炎严重程度的影响。C3 H/He小鼠在所有感染剂量下均发生严重关节炎，100%感染需要200个螺旋体。在BALB/cAnN小鼠中，关节炎的严重程度取决于感染剂量; 200 B的感染症状轻微。随着感染剂量的增加，病情逐渐加重。用2 × 10（4）B感染BALB/cAnN小鼠。burgdorferi导致关节炎，其严重程度与C3 H/He小鼠相同。PCR检测结果显示，C3 H/HeJ和C3 H/HeN小鼠后踝关节内螺旋体含量较高，且不随感染剂量的增加而增加。BALB/cAnN小鼠关节中的螺旋体水平随着感染剂量增加至严重关节炎C3 H/He小鼠中发现的水平而增加。因此，BALB/cAnN小鼠对严重关节炎的抵抗是有条件的：它可以通过高感染剂量克服，当高水平的B时，关节炎变得严重。在关节中存在Burgdorferi。在C57 BL/6 N小鼠中观察到对增加的感染剂量的独特反应，其在所有剂量的B下显示轻度至中度关节炎。burgdorferi测试，最多2 x 10（5）。在所有感染剂量下，C57 BL/6 N小鼠踝关节中的螺旋体水平均较高，与严重关节炎C3 H/He小鼠中发现的水平相当。在感染的（C57 BL/6 N x C3 H/HeN）F-1小鼠中观察到的关节炎的严重程度介于两个亲本品系之间。C57 BL/6 N小鼠对严重关节炎的抵抗力不能被高感染剂量克服，并且与关节中的螺旋体水平无关，这一发现表明，它是由与BALB/cAnN小鼠不同的机制介导的。
Studies of mice infected with Borrelia burgdorferi have indicated that the severity of arthritis is influenced by the genetic composition of the host: the C3H mouse develops severe arthritis while BALB/c and C57BL/6 mice develop mild arthritis. In this study, the effects of increasing infectious dose on the severity of arthritis were determined in these three mouse strains. C3H/He mice developed severe arthritis at all infectious doses, with 100% infection requiring 200 spirochetes. In BALB/cAnN mice, arthritis severity was dependent on infectious dose; symptoms were mild with infection by 200 B. burgdorferi and progressively more severe with increasing infectious dose. Infection of BALB/cAnN mice with 2 x 10(4) B. burgdorferi resulted in arthritis with severity identical to that in C3H/He mice. Spirochete levels in rear ankle joints of C3H/HeJ and C3H/HeN mice were relatively high, as detected by PCR, and did not increase with infectious dose. Spirochete levels in joints from BALB/cAnN mice increased,vith increasing infectious dose to levels found in severely arthritic C3H/He mice. Thus, resistance to severe arthritis in BALB/cAnN mice was conditional: it could be overcome by high infectious dose and the arthritis became severe when high levels of B. burgdorferi were present in joints. A unique response to increasing infectious dose was seen in C57BL/6N mice, which displayed mild to moderate arthritis at all doses of B. burgdorferi tested, up to 2 x 10(5). At all infectious doses, the levels of spirochetes in ankle joints of C57BL/6N mice were high, equivalent to those found in the severely arthritic C3H/He mice. The arthritis observed in infected (C57BL/6N x C3H/HeN)F-1 mice was of severity intermediate between those of the two parental strains. The finding that resistance to severe arthritis in C57BL/6N mice could not be overcome by high infectious doses and was independent of spirochete levels in joints suggested that it was mediated by a distinct mechanism from that operating in BALB/cAnN mice.