Cucumber CsBPCs Regulate the Expression of CsABI3 during Seed Germination.
Cucumber CsBPCs Regulate the Expression of CsABI3 during Seed Germination.
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DOI：
10.3389/fpls.2017.00459
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发表时间：
2017
影响因子：
5.6
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Mu Y;Liu Y;Bai L;Li S;He C;Yan Y;Yu X;Li Y
浅休眠的黄瓜种子在收获较晚的果实中开始发芽。脱落酸不敏感蛋白3（ABI 3）是脱落酸（阿坝）信号转导途径中的一个转录因子，是植物胚胎发育后期向萌发过渡的重要调控因子。我们的分析发现，在CsABI 3的启动子的黄瓜碱性五半胱氨酸（CsBPC）的候选顺式调控基序。酵母单杂交和染色质免疫沉淀（ChIP）分析表明，CsBPCs结合到CsABI 3的启动子。在过表达CsBPCs的转基因拟南芥植物中检测由CsABI 3启动子驱动的β-葡萄糖醛酸酶（GUS）活性和本氏烟草（烟草）荧光素酶测定表明CsBPCs抑制CsABI 3的表达。构建过表达CsBPCs的转基因植物以证实CsBPCs参与控制种子萌发。对黄瓜BPC-ABI 3信号通路的研究将有助于探索和表征种子萌发的分子机制，并为农林业种子保存提供必要的信息。
Cucumber seeds with shallow dormancy start to germinate in fruit that are harvested late. ABSCISIC ACID INSENSITIVE3 (ABI3), a transcription factor in the abscisic acid (ABA) signaling pathway, is one of the most important regulators in the transition from late embryogenesis to germination. Our analysis found a candidate cis-regulatory motif for cucumber BASIC PENTACYSTEINE (CsBPC) in the promoter of CsABI3. Yeast one-hybrid and chromatin immunoprecipitation (ChIP) assays showed that CsBPCs bound to the promoter of CsABI3. Examination of β-glucuronidase (GUS) activity driven by the CsABI3 promoter in transgenic Arabidopsis thaliana plants overexpressing CsBPCs and a Nicotiana benthamiana (tobacco) luciferase assay indicated that CsBPCs inhibited the expression of CsABI3. Transgenic plants overexpressing CsBPCs were constructed to confirm that CsBPCs participates in the control of seed germination. This study of the cucumber BPC-ABI3 pathway will help to explore and characterize the molecular mechanisms underlying seed germination and will provide necessary information for seed conservation in agriculture and forestry.