A NEW METHOD FOR THE PREPARATION OF ACYL-COA THIOESTERS
A NEW METHOD FOR THE PREPARATION OF ACYL-COA THIOESTERS
复制标题
DOI：
10.1093/oxfordjournals.jbchem.a133207
复制
发表时间：
1981-01-01
影响因子：
2.7
通讯作者：
OKUDA, S
中科院分区：
文献类型：
--
作者：
KAWAGUCHI, A;YOSHIMURA, T;OKUDA, S
介绍了一种制备辅酶A硫代酯的方法。以1-酰基咪唑为原料合成了酰基辅酶A硫酯，并用高效液相色谱对其进行了纯化。该方法适用于制备各种不同链长(从C2到C18)和不同不饱和度的酰基辅酶A硫酸酯。它特别适合于制备标记脂肪酸的酰基辅酶A硫代酯或仅有少量存在的脂肪酸。
A method is described for the preparation of CoA thioesters of fatty acids. Acyl-CoA thioester was synthesized by way of 1-acylimidazole and purified by high performance liquid chromatography. This method is applicable to the preparation of a variety of acyl-CoA thioesters of various chain lengths (from C2 to C18) and with varying degrees of unsaturation. It is particularly suitable for preparing acyl-CoA thioesters of labeled fatty acids or fatty acids available only in small amounts.