gamma-butyrobetaine hydroxylase: primary and secondary tritium kinetic isotope effects.
gamma-butyrobetaine hydroxylase: primary and secondary tritium kinetic isotope effects.
复制标题
γ-丁甜菜碱羟化酶：初级和次级氚动力学同位素效应。
DOI：
10.1021/bi00294a036
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发表时间：
1983
期刊：
影响因子：
2.9
通讯作者：
Englard,S
中科院分区：
文献类型：
--
作者：
Blanchard,JS;Englard,S
（Lindstedt 等，1970，1977）由 Goran Lindstedt 博士（瑞典哥德堡哥德堡大学）慷慨提供。经SDS-聚丙烯酰胺凝胶电泳测定，该酶是均质的，比活性为21单位/mg。小牛肝丁基甜菜碱羟化酶通过先前描述的离子交换和凝胶渗透色谱步骤纯化（Kondo 等人，1981）。这些酶制剂的活性范围在0.02至0.04单位/毫克之间；相比之下，如前所述，均质小牛肝酶的比活性为0.053单位/毫克（Kondo等，1981）。谷氨酸脱羧酶、酵母乙醇脱氢酶、乌头酸酶、异柠檬酸脱氢酶和谷氨酸脱氢酶购自Sigma Chemical Co.。NADP购自Boehringer-Mannheim。[14C]-甲基碘化物 (58 mCi/mmol) 购自 Amers-ham International Ltd。3H2G (100 mCi/mL) 和 4-氨基-[2, 3 (N)-3H] 丁酸酯 (27.2 Ci/mmol) 是 New England Nuclear 的产品。碘甲烷(puriss)和质子海绵1,8-双(二甲氨基)萘分别购自Tridom-Fluka和Aldrich Chemical Co.。所有其他化学品和试剂均具有市售最高纯度。
(Lindstedt et al., 1970, 1977), was generously provided by Dr. Goran Lindstedt (University of Gothenburg, Gothenburg, Sweden). The enzyme was homogeneous as determined by SDS-polyacrylamide gel electro-phoresis and had a specific activity of 21 units/mg. Calf liver-butyrobetaine hydroxylase was purified through the ion-exchange and gel-permeation chromatography steps previously described (Kondo et al., 1981). Theactivity of these enzyme preparations ranged between 0.02 and 0.04 unit/mg; by com-parison, the specific activity of the homogeneous calf liver enzyme was 0.053 unit/mg as previously reported (Kondo et al., 1981).Glutamate decarboxylase, yeast alcohol dehydrogenase, aconitase, isocitrate dehydrogenase, and glutamate dehydrogenase were purchased from Sigma Chemical Co. NADP was obtained from Boehringer-Mannheim.[14C]-Methyl iodide (58 mCi/mmol) was purchased from Amers-ham International Ltd. 3H2G (100 mCi/mL) and 4-amino-[2, 3 (N)-3H] butyrate (27.2 Ci/mmol) were products from New England Nuclear. Methyl iodide (puriss) and the proton sponge l, 8-bis (dimethylamino) naphthalene were purchased from Tridom-Fluka and Aldrich Chemical Co., respectively. All other chemicals and reagents were of the highest purity commercially avilable.
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利用氢同位素效应识别乙酸催化酮烯醇化反应中的攻击亲核试剂1-3
DOI：
--
发表时间：
1958
期刊：
影响因子：
--
作者：
C. G. Swain;E. C. Stivers;J. F. Reuwer;L. J. Schaad
通讯作者：
L. J. Schaad
影响因子：
2.9
作者：
GELB, MH;HEIMBROOK, DC;SLIGAR, SG
通讯作者：
SLIGAR, SG
来自假单胞菌 AK 1 的 γ-丁酰甜菜碱羟化酶。
影响因子：
2.9
作者：
G. Lindstedt;S. Lindstedt;M. Tofft
通讯作者：
M. Tofft
影响因子：
4.1
作者：
LABADIE, J;DUNN, WA;ARONSON, NN
通讯作者：
ARONSON, NN
影响因子：
15
作者：
B. Lindblad;G. Lindstedt;M. Tofft;S. Lindstedt
通讯作者：
S. Lindstedt