ANTIGEN MHC-SPECIFIC T-CELLS ARE PREFERENTIALLY EXPORTED FROM THE THYMUS IN THE PRESENCE OF THEIR MHC LIGAND
ANTIGEN MHC-SPECIFIC T-CELLS ARE PREFERENTIALLY EXPORTED FROM THE THYMUS IN THE PRESENCE OF THEIR MHC LIGAND
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DOI：
10.1016/0092-8674(89)90502-3
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发表时间：
1989-09-22
期刊：
影响因子：
64.5
通讯作者：
DAVIS, MM
中科院分区：
文献类型：
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作者：
BERG, LJ;PULLEN, AM;DAVIS, MM
已制备出表达鸽子细胞色素c片段特异性T细胞受体异二聚体和MHCⅱ类分子（I-Ek）的转基因小鼠。我们发现H-2k。转基因小鼠的T细胞数量总体增加，细胞色素c反应细胞的表达比H-2b小鼠高10倍。胸腺细胞表面染色表明，在H-2b小鼠中，T细胞发育被阻止在分化的中间阶段（CD4+8+, CD3lo）。携带这些T细胞受体基因和MHCⅱ类的小鼠分析。结构表明，这种发育阻滞可以通过胸腺皮质上皮细胞I-E的表达逆转。这些数据表明，在没有名义抗原的情况下，胸腺中发生了直接的T细胞受体- mhc相互作用，并导致T细胞输出增强，这与“积极选择”的概念一致。
Transgenic mice expressing a T cell receptor heterodimer specific for a fragment of pigeon cytochrome c plus an MHC class II molecule (I-Ek) have been made. We find that H-2k .alpha..beta. transgenic mice have an overall increase in the number of T cells and express a 10-fold higher fraction of cytochrome c-reactive cells than H-2b mice. Surface staining of thymocytes indicates that in H-2b mice, T cell development is arrested at an intermediate stage of differentiation (CD4+8+, CD3lo). Analyses of mice carrying these T cell receptor genes and MHC class II I-E.alpha. constructs indicate that this developmental block can be reversed in H-2b mice by I-E expression on cortical epithelial cells of the thymus. These data suggest that a direct T cell receptor-MHC interaction occurs in the thymus in the absence of nominal antigen and results in the enhanced export of T cells, consistent with the concept of "positive selection.".