Radiation suppresses neointimal hyperplasia through affecting proliferation and apoptosis of vascular smooth muscle cells
Radiation suppresses neointimal hyperplasia through affecting proliferation and apoptosis of vascular smooth muscle cells
复制标题
放射通过影响血管平滑肌细胞的增殖和凋亡来抑制新生内膜增生
DOI：
10.5301/jva.5000804
复制
发表时间：
2018-02
期刊：
影响因子：
--
通讯作者：
Lun Li
中科院分区：
文献类型：
--
作者：
Liqing Yuan;Chang shu;Xiao Zhou;Jiehua LI;Lunchang Wang;Li Xin;Xiang Xiong;Lun Li
目的：研究放射线对血管平滑肌细胞（VSMCs）的影响，探讨放射线预防人工血管移植物内膜增生的机制。材料和方法：在模型I中，12只杂种狗用人工移植物进行血运重建，一半狗接受28戈伊的移植物照射。在模型II中，维持人VSMCs（hVSMCs）并将其分为六组，分别施加六种不同剂量的外照射：0戈伊、2戈伊、8戈伊、16戈伊、24戈伊和30戈伊。在两种模型中，均采集标本，并采用形态学、免疫学、细胞和分子生物学方法进行检测。结果：照射后，照射组的新生内膜厚度显著降低（p ≤ 0.01）。放疗可上调p27kip 1表达，下调增殖细胞核抗原（PCNA）和S期激酶相关蛋白2（Skp 2）表达。X射线照射通过作用于细胞周期的G1/S期抑制hVSMCs的增殖。hVSMCs的凋亡率随剂量和时间的增加而显著增加。PCNA和Skp2的表达随照射剂量的增加呈先上升后下降的趋势，但与照射时间呈显著负相关。p27kip1的表达与剂量、时间呈显著正相关。结论：腹主动脉人工血管置换术后的术后外分割照射在短期内抑制了移植物新生内膜的增生。有一个显着的时间和剂量依赖性抑制VSMC增殖的辐射时，它是管理。
PURPOSE:.To study the effect of x-ray radiotherapy on vascular smooth muscle cells (VSMCs) and elucidate the mechanisms in preventing neointimal hyperplasia of prosthetic vascular grafts...MATERIALS AND METHODS:.In model I, twelve mongrel dogs underwent revascularization with prosthetic grafts and half the dogs underwent irradiation of the grafts at 28 Gy. In model II, human VSMCs (hVSMCs) were maintained and divided into six groups to which external radiation was applied at six different doses: 0 Gy, 2 Gy, 8 Gy, 16 Gy, 24 Gy and 30 Gy. In both models, specimens were harvested and examined by using morphological, immunological, cellular and molecular methods...RESULTS:.After irradiation, the neointima thickness was significantly lower in irradiated groups (p≤0.01). The radiotherapy could up-regulate p27kip1, and down-regulate proliferating cell nuclear antigen (PCNA) and S phase kinase associated protein 2 (Skp2). X-ray irradiation inhibits the proliferation of hVSMCs via acting on G1/S phase of cell cycle. The apoptosis of hVSMCs increased significantly with dose and time. The expression of PCNA and Skp2 were decreased after a first increasing trend with dose, but had a significant negative correlation with time. The expression of p27kip1 had a significant positive correlation with dose and time...CONCLUSIONS:.Postoperative external fractionated irradiation after prosthetic vessel replacement of the abdominal aorta suppressed the development of hyperplasia in the graft neointima in the short term. There was a prominent time- and dose-dependent inhibition of VSMC proliferation by radiation when it was administered.
登录
查看更多内容
DOI：
10.1161/atvbaha.111.235200
发表时间：
2012-01
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
Song GJ;Barrick S;Leslie KL;Bauer PM;Alonso V;Friedman PA;Fiaschi-Taesch NM;Bisello A
通讯作者：
Bisello A
影响因子：
5
作者：
Moreels, M.;Vandenabeele, F.;Lambrichts, I.
通讯作者：
Lambrichts, I.
影响因子：
158.5
作者：
Verin, V;Popowski, Y;Wijns, W
通讯作者：
Wijns, W
影响因子：
19.6
作者：
Misra, S.;Bonan, R.;Roy-Chaudhury, P.
通讯作者：
Roy-Chaudhury, P.
影响因子：
158.5
作者：
Leon, MB;Teirstein, PS;Kuntz, RE
通讯作者：
Kuntz, RE