Cloning and characterization of a cDNA encoding β-amyrin synthase from petroleum plant Euphorbia tirucalli L.
Cloning and characterization of a cDNA encoding β-amyrin synthase from petroleum plant Euphorbia tirucalli L.
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DOI：
10.1016/j.phytochem.2005.05.021
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发表时间：
2005-08-01
期刊：
影响因子：
3.8
通讯作者：
Ohyama, K
中科院分区：
文献类型：
--
作者：
Kajikawa, M;Yamato, KT;Ohyama, K
一品红被称为石油植物，能产生大量的三萜类化合物，如β-香草素。基于已知的β-香兰素合成酶保守序列的简并RT-PCR，克隆了一个可能的三萜类合成酶基因ETAS。其推导的氨基酸序列与人参β-淀粉蛋白合成酶的同源性最高，达82%。ETAS ORF在毕赤酵母中的异源表达导致了β-淀粉酶的产生，揭示了ETAS基因编码的是一种β-淀粉酶。这是大戟科植物中参与三萜类合成途径的基因的首次报道。(C)2005爱思唯尔有限公司。保留所有权利。
Euphorbia tirucalli L., known as the petroleum plant, produces a large amount of triterpenes, such as beta-amyrin. Degenerate RTPCR based on the sequences conserved among known beta-amyrin synthases led to cloning of a putative triterpene synthase cDNA, EtAS, from leaves of E tirucalli. The deduced amino acid sequence of the EtAS cDNA showed the highest identity of 82% to the Panax ginseng P-amyrin synthase. Heterologous expression of the EtAS ORF in the methylotrophic yeast, Pichia pastoris, resulted in production of beta-amyrin, revealing that the EtAS cDNA codes for a P-amyrin synthase. This is the first report of a gene involved in the triterpene synthetic pathway from Euphorbiaceae plants. (c) 2005 Elsevier Ltd. All rights reserved.