Evaluation of fluorescent polysaccharide nanoparticles for pH-sensing.
Evaluation of fluorescent polysaccharide nanoparticles for pH-sensing.
复制标题
DOI：
10.1039/b900260j
复制
发表时间：
2009-05
影响因子：
3.2
通讯作者：
A. Schulz;Stephanie Hornig;T. Liebert;E. Birckner;Thomas Heinze;Gerhard J Mohr
中科院分区：
文献类型：
--
作者：
A. Schulz;Stephanie Hornig;T. Liebert;E. Birckner;Thomas Heinze;Gerhard J Mohr
描述了新型葡聚糖纳米颗粒作为分析应用（例如生理学）的pH传感器的适用性的全面表征。用pH指示剂染料（异硫氰酸荧光素，FITC）和参考染料（磺酰罗丹明B酰氯）作为内标物标记纳米颗粒。FITC的荧光强度随着pH的增加而增加，而参比染料的信号保持恒定。绘制两个信号的比率与pH值的关系图，得出pK（a）为6.45，这适用于大多数测量目的。此外，温度，离子强度和氧化性物质对葡聚糖纳米颗粒内的荧光团的性能的影响进行了检查。将这些结果与溶解的染料进行比较，以评估葡聚糖基质是否影响传感器和参比染料的荧光性质，以及这些纳米传感器是否适用于生物样品中的pH传感。
The comprehensive characterization of novel dextran nanoparticles with regard to their suitability as pH-sensors for analytical applications (e.g. in physiology) is described. The nanoparticles are labeled with both a pH-indicator dye (fluorescein isothiocyanate, FITC) and a reference dye (sulforhodamine B acid chloride) as an internal standard. The fluorescence intensity of FITC increases with increasing pH, whereas the signal of the reference dye remains constant. Plotting the ratio of both signals against the pH gives a pK(a) of 6.45, which is appropriate for most of the measurement purposes. Furthermore, the influence of temperature, ionic strength and oxidizing substances on the performance of the fluorophores inside the dextran nanoparticles is examined. These results are compared to the dissolved dyes in order to evaluate if the dextran matrix affects the fluorescence properties of the sensor and the reference dye, and whether or not these nanosensors are suitable for pH-sensing in biological samples.