Effects of baicalein and wogonin isolated from Scutellaria baicalensis roots on skin damage in acute UVB-irradiated hairless mice
Effects of baicalein and wogonin isolated from Scutellaria baicalensis roots on skin damage in acute UVB-irradiated hairless mice
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DOI：
10.1016/j.ejphar.2011.04.033
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发表时间：
2011-07-01
影响因子：
5
通讯作者：
Sumiyoshi, Maho
中科院分区：
文献类型：
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作者：
Kimura, Yoshiyuki;Sumiyoshi, Maho
太阳紫外线（UV）辐射导致皮肤损伤，包括皮肤厚度增加、水肿和潮红。在这项研究中，我们研究了两个主要的黄酮类化合物（汉黄芩素和黄芩素）分离出的根黄芩，一个传统的治疗过敏性炎症性疾病的长期使用在中国和日本，对急性UVB辐射引起的无毛小鼠皮肤损伤的影响。黄芩素和汉黄芩素（10或50 mg/kg）每日两次口服，连续14天。在用两种主要黄酮类化合物处理的第7天和第8天，以200 mJ/cm（2）的剂量进行UVB照射。黄芩素和汉黄芩素可防止照射引起的皮肤厚度增加和基质金属蛋白酶（MMP）-9和VEGF水平的升高。汉黄芩素降低UVB处理的HaCaT细胞中环氧合酶（考克斯）-2和缺氧诱导因子（HIF）-1 α的水平。这些发现表明汉黄芩素通过抑制考克斯-2和HIF-1 α的表达来抑制MMP-9和VEGF水平的增加，从而抑制辐射诱导的皮肤损伤。黄芩素抑制考克斯-2和NF-κ B B/p65的表达，但刺激HIF-1 α的表达。因此，其抑制作用可能是由于通过抑制考克斯-2和NF-κ B/p65的表达来表达MMP-9和VEGF。黄芩素对UVB照射后皮肤表皮细胞增生的抑制作用可能与其刺激HIF-1 α的表达有关。(C)2011 Elsevier B. V.保留所有权利。
Solar ultraviolet (UV) radiation causes skin damage including increases in skin thickness, edema, and flush. In this study, we examined the effects of two main flavonoids (wogonin and baicalein) isolated from the roots of Scutellaria baicalensis, a traditional remedy for allergic inflammatory diseases long used in China and Japan, on acute UVB irradiation-induced skin damage in hairless mice. Baicalein and wogonin (10 or 50 mg/kg) were administered orally twice daily for 14 consecutive days. The UVB irradiation was performed at a dose of 200 mJ/cm(2) on days 7 and 8 of the treatment with the two main flavonoids. Baicalein, and wogonin prevented the increases in skin thickness and the levels of matrix metalloproteinase (MMP)-9, and vascular endothelial growth factor (VEGF) induced by the irradiation. Wogonin reduced the levels of cyclooxygenase (COX)-2 and hypoxia inducible factor (HIF)-1 alpha in UVB-treated HaCaT cells. These findings suggest that wogonin inhibits irradiation-induced skin damage by suppressing increases in the levels of MMP-9, and VEGF through the inhibition of COX-2 and HIF-1 alpha expression. Baicalein inhibited COX-2 and NF-kappa B/p65 expression, but stimulated HIF-1 alpha expression. Therefore, its inhibitory action is likely due to the expression of MMP-9 and VEGF through the suppression of COX-2 and NF-kappa B/p65 expression. Furthermore, the inhibitory effects of baicalein on UVB-irradiated hyperplasia of skin epidermis may be due to the stimulation of HIF-1 alpha expression. (C) 2011 Elsevier B.V. All rights reserved.