Fusarium solani is responsible for mass mortalities in nests of loggerhead sea turtle, Caretta caretta, in Boavista, Cape Verde.
Fusarium solani is responsible for mass mortalities in nests of loggerhead sea turtle, Caretta caretta, in Boavista, Cape Verde.
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茄镰孢是佛得角博阿维斯塔红海龟（Caretta caretta）巢穴中大量死亡的罪魁祸首。
DOI：
10.1111/j.1574-6968.2010.02116.x
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发表时间：
2010
影响因子：
2.1
通讯作者：
J. Diéguez
中科院分区：
文献类型：
--
作者：
J. M. Sarmiento;E. Abella;M. Martín;M. T. Telleria;L. López;A. Marco;J. Diéguez
茄病镰刀菌Fusarium solani（Mart.）Saccardo（1881年）被发现是佛得角博阿维斯塔岛海龟种Caretta caretta的卵感染的原因。从受感染的巢中取样具有早期和严重感染症状的蛋壳以及患病胚胎。获得了25个形态特征相似的菌株。其ITS rRNA基因序列与F. solani和它们的最大同一性范围为95%至100%。系统发育简约性和贝叶斯分析表明，这些分离物属于一个单一的F。solani分支，并分布在两个亚分支命名为A和C（后者包含23个25）。在挑战接种实验中使用亚进化枝C的代表性分离物来测试Koch假设。死亡率为C。攻毒组为83.3%，对照组为8.3%。接种的攻毒鸡蛋表现出与田间发现的感染鸡蛋相同的症状。因此，本工作表明，一组F。在海龟筑巢的海滩上观察到的症状是由solani引起的，它们对这一濒危物种的生存构成了风险。
The fungus Fusarium solani (Mart.) Saccardo (1881) was found to be the cause of infections in the eggs of the sea turtle species Caretta caretta in Boavista Island, Cape Verde. Egg shells with early and severe symptoms of infection, as well as diseased embryos were sampled from infected nests. Twenty-five isolates with similar morphological characteristics were obtained. Their ITS rRNA gene sequences were similar to the GenBank sequences corresponding to F. solani and their maximum identity ranged from 95% to 100%. Phylogenetic parsimony and Bayesian analyses of these isolates showed that they belong to a single F. solani clade and that they are distributed in two subclades named A and C (the latter containing 23 out of 25). A representative isolate of subclade C was used in challenge inoculation experiments to test Koch postulates. Mortality rates were c. 83.3% in challenged eggs and 8.3% in the control. Inoculated challenged eggs exhibited the same symptoms as infected eggs found in the field. Thus, this work demonstrates that a group of strains of F. solani are responsible for the symptoms observed on turtle-nesting beaches, and that they represent a risk for the survival of this endangered species.