Identification of Genes Encoding Atypical Odorant-Binding Proteins in Aedes albopictus (Diptera: Culicidae)
Identification of Genes Encoding Atypical Odorant-Binding Proteins in Aedes albopictus (Diptera: Culicidae)
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DOI：
10.1603/033.046.0211
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发表时间：
2009-03-01
影响因子：
2.1
通讯作者：
Zhao, Xiumei
中科院分区：
文献类型：
--
作者：
Armbruster, Peter;White, Sheryl;Zhao, Xiumei
昆虫气味结合蛋白（OBP）是一个多样化的基因家族，它编码的蛋白质被认为通过结合化学信息素并将其通过昆虫感器的水淋巴运输而发挥分子伴侣的作用。根据生物信息学标准，冈比亚按蚊 (Giles) 和埃及伊蚊中有 66 至 68 个基因被归类为 OBP。我们从白纹伊蚊 (Skuse) 的消减 cDNA 文库中克隆并测序了三个 OBP。 BLASTP 和推导的氨基酸序列的系统发育分析鉴定了 Ae 中独特的推定直向同源物。每个 Ae 对应埃及伊蚊。白纹伊蚊 OBP。这些假定的 Ae 的比较。埃及伊蚊直向同源物与先前对伊蚊中 OBP 基因的生物信息学分析结果。埃及伊蚊强调了生物信息学分析的潜在变异性，并表明库蚊的 OBP 基因家族比之前描述的更加多样化。推导的氨基酸序列比对和系统发育分析鉴定了与 aedine 特异性多样化相关的 Culicid OBP 的 N 末端区域。组织特异性表达分析表明，Ae 中的两个。白纹伊蚊 OBP 在成体前阶段和成体血淋巴中均表达，表明这些基因编码的蛋白质可能参与血淋巴中疏水性配体的转运。另一个艾。白纹伊蚊 OBP 仅在触角和腿中表达，表明其具有化学感应功能。这些结果在蚊子 OBP 的进化和功能多样化的背景下进行了讨论。
Insect odorant-binding proteins (OBPs) ire a diverse gene family that en code proteins thought to function as molecular chaperones by binding semiochemicals and transporting them through the aqueous lymph of insect sensilla. Between 66 and 68 genes have been classified as OBPs in both Anopheles gambiae (Giles) and Aedes aegypti L. based on bioninformatics criteria. We have cloned and sequenced from a subtracted cDNA library three OBPs in Aedes albopcitus (Skuse). BLASTP and phylogenetic analysis of deduced amino acid sequences identified a unique putative ortholog in Ae. aegypti for each Ae. albopictus OBP. Comparison of these putative Ae. aegypti orthologs with the results Of previous bioinformatics analyses of OBP genes in Ae. aegypti highlight the potential variability of bioinformatics analyses and Suggest that the OBP gene family of Culicids is even more diverse than previously described. Alignment of deduced amino acid sequences and phylogenetic analysis identified the N-terminal region of Culicid OBPs that is associated with aedine-specific diversification. Analysis of tissue-specific expression indicates that two of the Ae. albopictus OBPs are expressed both in preadult stages and in the hemolymph of adults, suggesting that the proteins encoded by these genes may be involved in the transport of hydrophobic ligands in the hemolymph. The other Ae. albopictus OBP is expressed exclusively in antennae and leg, suggesting a chemosensory function. These results ire discussed within the context of the evolution and functional diversification of OBPs in mosquitoes.