Matrix metalloproteinase-28 transcript and protein are expressed in rhesus monkey placenta during early pregnancy
Matrix metalloproteinase-28 transcript and protein are expressed in rhesus monkey placenta during early pregnancy
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DOI：
10.1093/molehr/gag028
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发表时间：
2003-04-01
影响因子：
4
通讯作者：
Zhu, C
中科院分区：
文献类型：
--
作者：
Li, QL;Illman, SA;Zhu, C
基质金属蛋白酶（matrix metalloproteinases， NIMP），尤其是新近发现的MNIP，在人类胚胎着床过程中滋养细胞侵袭中的作用尚不清楚。在本报告中，我们使用恒河猴早期妊娠模型，检测了最近发现的MNIP、MMP-28/epilysin、转录本和蛋白在妊娠第12、18和26天的恒河猴子宫样本中的表达和定位。妊娠第12天原位杂交显示MMP-28 mRNA的表达，胎盘绒毛的合胞层和细胞滋养层、滋养层柱的细胞滋养细胞和滋养层壳的外膜滋养细胞是MMP-28转录物的主要产生细胞。mmp - 28mrna在血管内滋养细胞、蜕细胞、管腔上皮和腺上皮、小动脉和肌层中均未检测到表达。RT-PCR分析在恒河猴第18天和第26天含有着床部位的子宫样本中扩增出258个核苷酸片段。经测序证实，该cDNA片段是MMP-28的血红素样结构域的一部分。与人MMP-28基因对应区域具有95%的同源性。免疫组化分析进一步证实MMP-28蛋白的定位与此相似。它的信使rna这种新型NIMP在恒河猴妊娠早期绒毛和绒毛外滋养层细胞中的有限分布模式提示其在胚胎着床过程中与滋养层细胞侵袭有关的潜在作用。
The role of matrix metalloproteinases (NIMP), especially newly described MNIP, in trophoblast invasion during human embryo implantation is poorly understood. In this report, using a model of early pregnancy in the rhesus monkey, we have examined the expression and localization of the most recently identified MNIP, MMP-28/epilysin, transcript and protein in macaque uterine samples on days 12, 18 and 26 of pregnancy. MMP-28 mRNA expression was shown by in-situ hybridization after day 12 of pregnancy, and both the syncytial and the cytotrophoblastic cell layers of placental villi, the cytotropboblast cells of the trophoblastic column, and the extravillous trophoblast cells of trophoblastic shell were primary producers of MMP-28 transcript. Expression of MMP-28 mRNA was undetectable in the endovascular trophoblast cells, decidual cells, luminal and glandular epithelium, arterioles, and myometrium. RT-PCR analysis amplified a fragment of 258 nucleotides from rhesus monkey uterine samples containing implantation sites on days 18 and 26. The cDNA fragment, following sequencing, was confirmed to be part of the haemopexin-like domain of MMP-28. It has 95% identity with the corresponding region of human MMP-28 gene. Immunohistochemical analysis further demonstrated that the localization of MMP-28 protein was similar to that. of its mRNA. The restricted distribution pattern of this novel NIMP in the villous and extravillous trophoblasts during rhesus monkey early pregnancy suggests a potential role in trophoblast invasion associated with embryo implantation.