Detection of substance P and its receptor in human fetal microglia
Detection of substance P and its receptor in human fetal microglia
复制标题
DOI：
10.1016/s0306-4522(00)00398-5
复制
发表时间：
2000-01-01
期刊：
影响因子：
3.3
通讯作者：
Ho, WZ
中科院分区：
文献类型：
--
作者：
Lai, JP;Zhan, GX;Ho, WZ
P物质是中枢神经系统中最丰富的神经激肽，是免疫系统的主要调节剂。我们检测了P物质及其受体在人胎儿脑小胶质细胞中的基因表达。采用逆转录聚合酶链反应和Southern印迹法，检测小胶质细胞中α、β、γ和δ四种前原激肽- a基因转录物。通过酶免疫测定，人胎儿小胶质细胞产生的内源性P物质蛋白水平（640-850 pg/10(6)个细胞）明显高于人外周血单核细胞来源的巨噬细胞（25-50pg/10(6)个细胞）。采用抗P物质抗体免疫组化染色，观察细胞膜P物质免疫反应性。此外，我们鉴定了神经激肽-1受体信使RNA的存在，神经激肽-1受体是人类胎儿小胶质细胞中P物质的主要受体。根据这些数据，我们提出P物质及其受体在生物学上参与调节小胶质细胞的功能，并可能在中枢神经系统的宿主防御中发挥重要作用。(c) 2000 ibro。Elsevier Science Ltd.出版。版权所有。
Substance P, the most abundant neurokinin in the CNS, is a major modulator of the immune system. We have examined the gene expression of substance P and its receptor in human fetal brain microglia. Using reverse transcription-polymerase chain reaction and Southern blotting assay, the four isoforms of preprotachykinin-A gene transcripts (alpha, beta, gamma and delta) were detected in the microglia. The human fetal microglia produced significantly higher levels of endogenous substance P protein (640-850 pg/10(6) cells) than did human peripheral blood monocyte-derived macrophages (25-50pg/10(6) cells), as determined by an enzyme immunoassay. Using immunohistochemical staining with an anti-substance P antibody, cell membrane substance P immunoreactivity was observed. In addition, we identified the presence of messenger RNA for neurokinin-1 receptor, a primary receptor for substance P in human fetal microglia.From these data, we propose that substance P and its receptor are biologically involved in regulating the functions of microglia, and potentially play an important role in host defense of the central nervous system. (C) 2000 IBRO. Published by Elsevier Science Ltd. All rights reserved.