Protective effects of α-tocopherol and ascorbic acid against cardol-induced cell death and reactive oxygen species generation in Staphylococcus aureus.
Protective effects of α-tocopherol and ascorbic acid against cardol-induced cell death and reactive oxygen species generation in Staphylococcus aureus.
复制标题
α-生育酚和抗坏血酸对金黄色葡萄球菌中卡多尔诱导的细胞死亡和活性氧生成的保护作用。
DOI：
10.1055/s-0032-1328555
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Fujita K.
中科院分区：
文献类型：
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作者：
Murata W;Tanaka T;Kubo I;Fujita K.
强心酚（C15：3），分离自腰果（腰果属 （octagentale L.）坚果壳液体，已经显示出 对各种葡萄球菌菌株的杀菌活性 金黄色葡萄球菌，包括耐甲氧西林菌株。最大水平 检测到活性氧产生的最小值 强心酚的杀菌浓度，而活性氧 当剂量高于最低杀菌剂量时， 浓度.对周围细菌活性的主要反应是 注意到杀菌浓度主要来源于氧化 应激如细胞内活性氧物质产生。高剂量 强心酚（C15：3）诱导S. 金黄色细胞，这可能与减少， 活性氧抗氧化剂如α-生育酚和 抗坏血酸抑制活性氧的产生， 脂质引起的细胞损伤。强心（C15：3）过量 可能会破坏天然膜相关功能，因为它作为一个 表面活性Cardols对S. aureus取决于其logP值（分配系数 辛醇/水），并与它们与漆树的相似性有关。 从同一来源分离的酸。
Cardol (C15 : 3), isolated from cashew(Anacardium occidentaleL.) nut shell liquid, has been shown to exhibit bactericidal activity against various strains ofStaphylococcus aureus,including methicillin-resistant strains. The maximum level of reactive oxygen species generation was detected at around the minimum bactericidal concentration of cardol, while reactive oxygen species production drastically decreased at doses above the minimum bactericidal concentration. The primary response for bactericidal activity around the bactericidal concentration was noted to primarily originate from oxidative stress such as intracellular reactive oxygen species generation. High doses of cardol (C15 : 3) were shown to induce leakage of K+fromS. aureuscells, which may be related to the decrease in reactive oxygen species. Antioxidants such asα-tocopherol and ascorbic acid restricted reactive oxygen species generation and restored cellular damage induced by the lipid. Cardol (C15 : 3) overdose probably disrupts the native membrane-associated function as it acts as a surfactant. The maximum antibacterial activity of cardols againstS. aureusdepends on their logPvalues (partition coefficient in octanol/water) and is related to their similarity to those of anacardic acids isolated from the same source.