Aqueous proinflammatory cytokines in acute primary angle-closure eyes
Aqueous proinflammatory cytokines in acute primary angle-closure eyes
复制标题
急性原发性闭角眼中的水性促炎细胞因子
DOI：
10.18240/ijo.2017.05.12
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发表时间：
2017-05-18
影响因子：
1.4
通讯作者：
Zhang, Xiu-Lan
中科院分区：
文献类型：
--
作者：
Liu, Yao-Ming;Chen, Shi-Da;Zhang, Xiu-Lan
目的：评价急性原发性闭角型（APAC）和年龄相关性白内障患者房水中促炎细胞因子的变化。方法：本横断面研究纳入20名APAC患者的20只眼和15名年龄相关性白内障患者的15只眼。前瞻性地收集房水样本。使用多重微珠免疫测定技术评估了 APAC 和白内障患者房水中 20 种促炎细胞因子的水平。收集临床数据进行相关分析。 结果：磁珠组中包含的 20 种促炎细胞因子中有 7 种在 APAC 眼和白内障眼中均可检测到：白细胞介素 (IL)-10、IL-12、IL-15、IL-21、IL-6、趋化因子（C-C 基序）配体 20 和肿瘤坏死因子 α (TNF-α)。 IL-27 仅在亚太地区的眼睛中可检测到。与白内障眼相比，APAC 眼的 IL-12 (P=0.036)、IL-15 (P=0.001)、IL-6 (P=0.012) 和 IL-27 浓度显着升高（仅在 APAC 眼中检测到）。年龄与 IL-12 (P=0.022) 和 IL-6 (P=0.037) 呈正相关，APAC 发病和房水样本采集之间的时间间隔与 IL-15 (P=0.037)、IL-27 (P=0.040) 和 TNF-α (P=0.042) 呈正相关。 包括 IL-12、IL-15、IL-6 和 IL-27 在内的细胞因子在 APAC 眼中升高，可能与其病理机制有关。
AIM: To evaluate changes of proinflammatory cytokines in aqueous humor of patients with acute primary angle-closure (APAC) and age-related cataracts.METHODS: Twenty eyes of 20 APAC patients and 15 eyes of 15 age-related cataract patients were included in this cross-sectional study. Aqueous humor samples were collected prospectively. The levels of 20 proinflammatory cytokines were evaluated in the aqueous humor of the APAC and cataract patients using the multiplex bead immunoassay technique. Clinical data were collected for correlation analysis.RESULTS: Seven of the 20 proinflammatory cytokines included in the magnetic bead panel were detectable in both APAC eyes and cataract eyes: interleukin (IL)-10, IL-12, IL-15, IL-21, IL-6, chemokine (C-C motif) ligand 20, and tumor necrosis factor alpha (TNF-alpha). IL-27 was only detectable in APAC eyes. Compared with the cataract eyes, the APAC eyes had significantly elevated concentrations of IL-12 (P=0.036), IL-15 (P=0.001), IL-6 (P=0.012), and IL-27 (only detectable in APAC eyes). Age was positively correlated with IL-12 (P=0.022) and IL-6 (P=0.037), and time elapsed between APAC onset and aqueous humor samples collection was positively correlated with IL-15 (P=0.037), IL-27 (P=0.040), and TNF-alpha (P=0.042).CONCLUSION: Several proinflammatory cytokines including IL-12, IL-15, IL-6 and IL-27, were elevated in the APAC eyes and may be implicated in its pathologic mechanism.