Influence of nanotopography on periodontal ligament stem cell functions and cell sheet based periodontal regeneration.
Influence of nanotopography on periodontal ligament stem cell functions and cell sheet based periodontal regeneration.
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纳米形貌对牙周膜干细胞功能和基于细胞片的牙周再生的影响
DOI：
10.2147/ijn.s83357
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发表时间：
2015
影响因子：
8
通讯作者：
Jin Y
中科院分区：
文献类型：
--
作者：
Gao H;Li B;Zhao L;Jin Y
牙周膜再生是再生医学的重要组成部分，具有重要的临床意义;然而，纳米形貌对牙周膜（PDL）干细胞（PDLSC）功能和基于PDLSC片的牙周再生的影响从未被探索过。钛（Ti）上的二氧化钛纳米管（NTs）提供了一个很好的平台来研究这一点。本研究观察了不同管径的NTs对PDLSCs功能的影响。然后，异位种植模型，使用钛/细胞片/羟基磷灰石（HA）复合物被施加到研究的影响，NT细胞片为基础的牙周再生。NT能够增强初始PDLSC粘附和扩散以及胶原分泌。利用Ti/细胞片/HA复合物模型，证明了当与骨髓间充质干细胞（BMSC）片和HA组合时，PDLSC片能够再生PDL组织，而不需要额外的可溶性化学线索。同时，NTs改善了异位植入的Ti/细胞片/HA复合物的牙周再生结果，产生了功能性排列的胶原纤维束。具体而言，在NT 5和NT 10样品组中观察到更致密的胶原纤维，在Ti表面上具有丰富的血管以及骨水泥样组织，其与天然PDL的结构非常相似。我们的研究首次证明了纳米形貌线索明显影响PDLSC的功能，并依赖性地提高PDLSC片基牙周再生的大小，这为牙周再生提供了新的见解。Ti/cell sheets/HA复合体可作为预测生物材料促进牙周组织再生的良好模型。
Periodontal regeneration is an important part of regenerative medicine, with great clinical significance; however, the effects of nanotopography on the functions of periodontal ligament (PDL) stem cells (PDLSCs) and on PDLSC sheet based periodontal regeneration have never been explored. Titania nanotubes (NTs) layered on titanium (Ti) provide a good platform to study this. In the current study, the influence of NTs of different tube size on the functions of PDLSCs was observed. Afterward, an ectopic implantation model using a Ti/cell sheets/hydroxyapatite (HA) complex was applied to study the effect of the NTs on cell sheet based periodontal regeneration. The NTs were able to enhance the initial PDLSC adhesion and spread, as well as collagen secretion. With the Ti/cell sheets/HA complex model, it was demonstrated that the PDLSC sheets were capable of regenerating the PDL tissue, when combined with bone marrow mesenchymal stem cell (BMSC) sheets and HA, without the need for extra soluble chemical cues. Simultaneously, the NTs improved the periodontal regeneration result of the ectopically implanted Ti/cell sheets/HA complex, giving rise to functionally aligned collagen fiber bundles. Specifically, much denser collagen fibers, with abundant blood vessels as well as cementum-like tissue on the Ti surface, which well-resembled the structure of natural PDL, were observed in the NT5 and NT10 sample groups. Our study provides the first evidence that the nanotopographical cues obviously influence the functions of PDLSCs and improve the PDLSC sheet based periodontal regeneration size dependently, which provides new insight to the periodontal regeneration. The Ti/cell sheets/HA complex may constitute a good model to predict the effect of biomaterials on periodontal regeneration.