Farp2 and Stk25 are candidate genes for the HDL cholesterol locus on mouse chromosome 1.
Farp2 and Stk25 are candidate genes for the HDL cholesterol locus on mouse chromosome 1.
复制标题
DOI：
10.1161/atvbaha.108.178384
复制
发表时间：
2009-01
期刊：
影响因子：
--
通讯作者：
Paigen B
中科院分区：
文献类型：
--
作者：
Su Z;Cox A;Shen Y;Stylianou IM;Paigen B
To identify the gene responsible for the quantitative trait locus (QTL) Hdlq14, a high density lipoprotein cholesterol (HDL) QTL previously identified in a C57BL/6J x 129S1/SvImJ cross. Hdlq14 was first confirmed as an independent QTL by detecting it in an intercross between NZB/B1NJ and NZW/LacJ, two strains that had identical genotypes at nearby QTL genes on chromosome 1. Using the bioinformatics tools of combined cross data and haplotype analysis, we narrowed this QTL from a 45 Mb, 225 -gene region to 2 genes, Farp2 and Stk25. Sequencing and expression studies showed that Farp2 had an amino acid polymorphism in an important plekstrin domain and that Stk25 had a significant expression difference between the parental strains. These two genes are immediately adjacent to each other and share the same haplotype over 45 inbred strains. The haplotype was associated with a significant difference in HDL levels among these strains. We confirmed Hdlq14 as a separate independent QTL for HDL and narrowed the region to two genes, Farp2 and Stk25 with considerable evidence for both. Additional studies are needed to choose between these two genes or to show that both are important in determining HDL levels.
