PsRPs26, a 40S Ribosomal Protein Subunit, Regulates the Growth and Pathogenicity of Puccinia striiformis f. sp. Tritici
PsRPs26, a 40S Ribosomal Protein Subunit, Regulates the Growth and Pathogenicity of Puccinia striiformis f. sp. Tritici
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DOI：
10.3389/fmicb.2019.00968
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发表时间：
2019-05
影响因子：
5.2
通讯作者：
Bing Wang;N. Song;Chunlei Tang;Jinbiao Ma;N. Wang;Yanfei Sun;Z. Kang
中科院分区：
文献类型：
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作者：
Bing Wang;N. Song;Chunlei Tang;Jinbiao Ma;N. Wang;Yanfei Sun;Z. Kang
真核核糖体对于细胞增殖、分化和生长是必需的。RPs 26是一种核糖体亚基结构蛋白，参与生长发育过程。PsRPs 26在病原真菌中的功能尚不清楚。本研究从小麦条锈菌（Puccinia striiformis f.）小麦种（Pst）PsRPs 26含有一个真核生物特异性的Y 62-K70基序，与其他真菌的同源基因有90%以上的同源性。发现PsRPs 26定位于细胞核和细胞质中。当小麦幼苗接种Pst CYR 31分离物时，PsRPs 26的表达增加。此外，敲低PsRPs 26由主机诱导的基因沉默系统抑制生长和限制夏孢子生产Pst。PsRPs 26的发现可能与Pst的致病性有关，为研究PsRPs 26在禾谷锈菌中的致病功能开辟了新途径。
Eukaryotic ribosomes are essential for proliferation, differentiation, and cell growth. RPs26 is a ribosomal subunit structural protein involved in the growth and development process. Little is known about the function of PsRPs26 in pathogenic fungi. In this study, we isolated the RPs26 gene, PsRPs26, from Puccinia striiformis f. sp. tritici (Pst). PsRPs26 contains a eukaryotic-specific Y62–K70 motif and is more than 90% identical with its ortholog gene in other fungi. PsRPs26 was found to be localized in both the nucleus and cytoplasm. Expression of PsRPs26 increased when wheat seedlings were inoculated with the Pst CYR31 isolate. Moreover, knockdown of PsRPs26 by a host-induced gene silencing system inhibited growth and limited urediospore production in Pst. Our discovery that PsRPs26 may contribute to the pathogenicity of Pst and open a new way in the pathogenic function of PsRPs26 in cereal rust fungi.