DNA copy number aberrations associated with the clinicopathological features of colorectal cancers: Identification of genomic biomarkers by array-based comparative genomic hybridization
DNA copy number aberrations associated with the clinicopathological features of colorectal cancers: Identification of genomic biomarkers by array-based comparative genomic hybridization
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DOI：
10.3892/or.2011.1246
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发表时间：
2011-06-01
期刊：
影响因子：
4.2
通讯作者：
Sasaki, Kohsuke
中科院分区：
文献类型：
--
作者：
Nakao, Motonao;Kawauchi, Shigeto;Sasaki, Kohsuke
本研究的目的是通过基于阵列的比较基因组杂交(CGH)来研究与结直肠癌(CRC)的关键临床病理特征和预后相关的染色体异常。应用细菌人工染色体(BAC)全基因组共4030个克隆，以0.83兆碱基对的平均间隔，对94例结直肠癌新鲜冰冻肿瘤组织进行了分析。DNA拷贝数变异(DCNA)与临床病理特征相关：9q33.1和20p12.2的缺失和8q24.3的缺失与淋巴结转移有关，与分期相关的8q24.3和9q33.1的缺失，与淋巴管侵犯的3p25.1、10p15.3、12q15和17p13.1的缺失与8q21.11和10q21.3的缺失有关。这些异常可被视为预测结直肠癌患者临床结果的基因组生物标志物，并有望服务于结直肠癌患者的个体化治疗。
The aim of the present study was to investigate the chromosomal aberrations that are linked with the crucial clinicopathological features of colorectal cancer (CRC) and its prognosis by array-based comparative genomic hybridization (CGH). Fresh-frozen tumor tissues of 94 cases of CRC were analyzed by using bacterial artificial chromosome (BAC) CGH slides spotted with 4030 human BAC clones, which covered the whole range of the human genome at an average interval of 0.83 mega base pairs. DNA copy number aberrations (DCNAs) were identified in association with clinicopathological features: a gain of 8q24.3 and losses of 9q33.1 and 20p12.2 were associated with lymph node metastasis, gain of 8q24.3 and loss of 9q33.1 with disease stage, gain of 8q21.11 and loss of 10q21.3 with lymphovascular invasion and losses of 3p25.1, 10p15.3, 12q15 and 17p13.1 for venous invasion. These aberrations can be regarded as genomic biomarkers to predict the clinical outcome of patients with CRC, and are expected to serve to individualize the treatment of CRC patients.