Long-distance communication in synthetic bacterial consortia through active signal propagation
Long-distance communication in synthetic bacterial consortia through active signal propagation
复制标题
通过主动信号传播在合成细菌群落中进行长距离通信
DOI：
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
R. Murray
中科院分区：
文献类型：
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作者：
James Parkin;R. Murray
理想的合成细胞-细胞信号通路应该是（1）代谢轻量型的，（2）与内源性基因网络绝缘，（3）可兴奋的，而不是振荡的或振荡的。为了实现这三个功能，我们提出了一个同步脉冲产生电路的基础上发表的同步振荡器的设计。该通信模块采用了一个脉冲发生器，使用Lux-type群体感应组件和IFFL转录电路。这个模块的输入和输出都是AHLs，即群体感应信号分子。因此，携带该模块的细胞充当可兴奋介质，当受到外源性阿勒刺激时产生阿勒脉冲。使用模拟和显微镜，我们演示了这个电路如何使旅行脉冲阿勒生产通过小菌落生长在两个维度。行进脉冲在比通过信号从发送者到接收者细胞的扩散所能实现的更长的距离上实现细胞间通信，并且可以允许合成联合体中更复杂的协调。
A synthetic cell-cell signaling circuit should ideally be (1) metabolically lightweight, (2) insulated from endogenous gene networks, and (3) excitable rather than oscillatory or bistable. To accomplish these three features, we propose a synchronized pulse-generating circuit based on the design of published synchronized oscillators. This communication module employs a pulse generator built using Lux-type quorum sensing components and an IFFL transcriptional circuit. Both the input and output of this module are AHLs, the quorum sensing signaling molecule. Cells bearing this module therefore act as an excitable medium, producing a pulse of AHL when stimulated by exogenous AHL. Using simulation and microscopy, we demonstrate how this circuit enables traveling pulses of AHL production through microcolonies growing in two dimensions. Traveling pulses achieve cell-cell communication at longer distances than can be achieved by diffusion of signal from sender to receiver cells and may permit more sophisticated coordination in synthetic consortia.