Analysis of isothiocyanates in newly generated vegetables, Baemuchae (xBrassicoraphanus) as affected by growth
Analysis of isothiocyanates in newly generated vegetables, Baemuchae (xBrassicoraphanus) as affected by growth
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DOI：
10.1111/j.1365-2621.2009.01970.x
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发表时间：
2009-07-01
影响因子：
3.3
通讯作者：
Kim, Jong-Kee
中科院分区：
文献类型：
--
作者：
Lim, Sooyeon;Lee, Joonhee;Kim, Jong-Kee
芸苔属植物中的异硫氰酸酯表现出与诱导II相解毒酶相关的抗癌特性。目前，异硫氰酸酯在萝卜（Raphanus sativus L.）和大白菜（Brassica campestris L.）SSP. pekinensis）。采用高效液相色谱-蒸发光散射检测器和气相色谱-质谱联用技术，对制备植物中的萝卜硫素和萝卜硫素等异硫氰酸酯进行了定性和定量分析。萝卜硫素构成了Baemuchae和萝卜中的主要异硫氰酸盐，而萝卜硫素则在西兰花中被检测到。萝卜硫素含量在花椰菜生长过程中变化，从芽中的144.9 +/- 8.69下降到嫩叶中的8.1 +/- 1.07 μ g g（-1）。在Baemuchae和萝卜芽中的磺胺草芬含量分别为172.6 +/- 11.23和131.3 +/- 4.08 μ g g g（-1），很少有。受植物生长的影响。研究结果表明，新产生的农作物白木樨中含有对甲砜吩，具有作为功能材料利用的潜力。
Isothiocyanates in Brassica exhibit anticancer properties related to induction of phase II detoxification enzymes. Currently, variations of isothiocyanates was evaluated in Baemuchae (XBrassicoraphanus), which was newly generated by inter-specific hybrid crops between radish (Raphanus sativus L.) and Chinese cabbage (Brassica campestris L. ssp. pekinensis). A high-performance liquid chromatography system coupled with an evaporative light scattering detector and gas chromatography-mass spectrometry were utilised to identify and quantify isothiocyanates including sulforaphene and sulforaphane in prepared plants. Sulforaphene constituted a major isothiocyanate in Baemuchae and radish, while sulforaphane was detected in broccoli. Sulforaphane contents varied during broccoli growth, with a constant decrease noted from 144.9 +/- 8.69 in sprouts to 8.1 +/- 1.07 mu g g(-1) in young leaves. Sulforaphene contents in Baemuchae and radish sprouts were 172.6 +/- 11.23 and 131.3 +/- 4.08 mu g g(-1), respectively, and were rarely a. ected by plant growth. The result supported that newly generated crop Baemuchae had potential to be utilised as functional material because of presence of sulforaphene.