Gene expression plasticity in response to salinity acclimation in threespine stickleback ecotypes from different salinity habitats
Gene expression plasticity in response to salinity acclimation in threespine stickleback ecotypes from different salinity habitats
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DOI：
10.1111/mec.14065
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发表时间：
2017-05-01
影响因子：
4.9
通讯作者：
Schulte, Patricia M.
中科院分区：
文献类型：
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作者：
Gibbons, Taylor C.;Metzger, David C. H.;Schulte, Patricia M.
表型可塑性被认为有助于新环境的殖民化，并塑造殖民种群的进化方向。然而，相对流行的各种预测模式的变化表型可塑性殖民化后仍不清楚。在这里，我们使用一个全转录组的方法来表征模式的基因表达可塑性的鳃的淡水适应和咸水适应的生态型threespine棘鱼（Gasterosteus aculeatus）暴露于一系列的盐度。鳃转录组对环境盐度的响应有一个共同的两个生态型（2159个基因）的大共享组件，具有显着富集的基因参与跨膜离子转运和鳃上皮细胞的重组。这种对淡水适应的转录反应在盐度低于千分之二时被诱导。也有分化的基因表达模式之间的生态型（2515个基因），特别是在鳃结构和渗透性的变化重要的过程。只有508个不同的生态型之间的基因也响应于盐度，并没有特定的过程中，这个基因集的丰富，甚至更少的数量（87个基因）显示的证据表明，在不同的生态型之间的盐度驯化的反应程度的变化。没有模式的相对表达占主导地位，这些基因，这表明，既不增益或损失的可塑性占主导地位的生态型之间的表达模式的变化。这些数据表明，多种模式的基因表达可塑性的变化可能会发生以下新的栖息地殖民。
Phenotypic plasticity is thought to facilitate the colonization of novel environments and shape the direction of evolution in colonizing populations. However, the relative prevalence of various predicted patterns of changes in phenotypic plasticity following colonization remains unclear. Here, we use a whole-transcriptome approach to characterize patterns of gene expression plasticity in the gills of a freshwater-adapted and a saltwater-adapted ecotype of threespine stickleback (Gasterosteus aculeatus) exposed to a range of salinities. The response of the gill transcriptome to environmental salinity had a large shared component common to both ecotypes (2159 genes) with significant enrichment of genes involved in transmembrane ion transport and the restructuring of the gill epithelium. This transcriptional response to freshwater acclimation is induced at salinities below two parts per thousand. There was also differentiation in gene expression patterns between ecotypes (2515 genes), particularly in processes important for changes in the gill structure and permeability. Only 508 genes that differed between ecotypes also responded to salinity and no specific processes were enriched among this gene set, and an even smaller number (87 genes) showed evidence of changes in the extent of the response to salinity acclimation between ecotypes. No pattern of relative expression dominated among these genes, suggesting that neither gains nor losses of plasticity dominated the changes in expression patterns between the ecotypes. These data demonstrate that multiple patterns of changes in gene expression plasticity can occur following colonization of novel habitats.