In-vitro Culture with a Tilting Device in Chemically Defined Media During Meiotic Maturation and Early Development Improves the Quality of Blastocysts Derived from In-vitro Matured and Fertilized Porcine Oocytes
In-vitro Culture with a Tilting Device in Chemically Defined Media During Meiotic Maturation and Early Development Improves the Quality of Blastocysts Derived from In-vitro Matured and Fertilized Porcine Oocytes
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DOI：
10.1262/jrd.10-041h
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发表时间：
2010-10-01
影响因子：
1.8
通讯作者：
Funahashi, Hiroaki
中科院分区：
文献类型：
--
作者：
Koike, Takayuki;Matsuura, Koji;Funahashi, Hiroaki
在生理条件下，哺乳动物卵母细胞和胚胎似乎不仅受到化学刺激，而且受到机械刺激，例如通过卵泡和雌性生殖道中的压缩、剪切应力和/或摩擦力。本研究旨在探讨猪卵母细胞体外成熟（IVM）和体外受精（IVF）后的体外培养（IVC）对早期发育能力和囊胚质量的影响。在含有促性腺激素和双丁酰cAMP的改良猪卵母细胞培养基（mPOM）中培养20 h后，倾斜培养的卵丘-卵母细胞复合体（COCs）的平均直径大于静态培养，而卵母细胞的直径没有差异。当培养的COCs继续另外在一个新鲜的培养基中没有促性腺激素和二丁酰cAMP为24小时，卵母细胞完成GVBD和发展到中期II阶段的发生率之间的倾斜和静态培养系统没有差异。此外，体外受精后的精子穿透和卵母细胞的发育能力的囊胚期之间的倾斜和静态系统在IVM和IVC没有什么不同。但IVM和IVC倾斜培养对每个囊胚的细胞数有显著的正效应（P
Under physiological conditions, mammalian oocytes and embryos appear to be stimulated not only chemically but also mechanically, such as by compression, shear stress and/or friction force in the follicle and female reproductive tract. The present study was undertaken to examine the effects of kinetic culture with a tilting device in chemically defined media during in vitro maturation (IVM) of porcine oocytes and in vitro culture (IVC) following in vitro fertilization (IVF) on the early developmental competence and quality of blastocysts. After culture in a chemically defined IVM medium, modified porcine oocyte medium (mPOM) containing gonadotropins and dibutyryl cAMP for 20 h, the mean diameter of the cumulus-oocyte complexes (COCs) was larger in the tilting culture than in the static controls, whereas the diameter of the oocytes did not differ. When culture of the COCs was continued additionally in a fresh medium without gonadotropins and dibutyryl cAMP for 24 h, the incidences of oocytes completing GVBD and developing to the metaphase-II stage did not differ between the tilting and static culture systems. Furthermore, the sperm penetration after IVF and developmental competence of the oocytes to the blastocyst stage were not different between the tilting and static systems during IVM and IVC. However, tilting culture during both IVM and IVC had a significant positive effect on the number of cells per blastocyst (P