Chitosan derivatives killed bacteria by disrupting the outer and inner membrane
Chitosan derivatives killed bacteria by disrupting the outer and inner membrane
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DOI：
10.1021/jf061310p
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发表时间：
2006-09-06
影响因子：
6.1
通讯作者：
Kim, Se-Kwon
中科院分区：
文献类型：
--
作者：
Je, Jae-Young;Kim, Se-Kwon
通过在壳聚糖C-6位接枝氨基官能团，制备了6种水溶性壳聚糖，并研究了它们对3种革兰氏阴性菌和3种革兰氏阳性菌的抗菌活性。在这些衍生物中，二甲基氨基乙基壳聚糖（DMAEC）具有最高的抑制细菌生长的潜力。为了阐明针对细菌的详细抗菌作用模式，研究了细胞完整性、外膜（OM）和内膜（IM）透化试验。当用DMAEC处理时，大肠杆菌和金黄色葡萄球菌的260 nm吸收材料的释放迅速增加，但由于细胞结构的差异，吸光度值不同。在OM和IM透化试验中，DMAEC通过增加OM和IM的渗透性迅速增加1-N-苯基萘胺的摄取和细胞质β-半乳糖苷酶的释放。结果表明，90%脱乙酰壳聚糖制备的DMAEC 90比50%脱乙酰壳聚糖制备的DMAEC 50具有更高的抑菌活性，说明水溶性壳聚糖的抑菌活性与脱乙酰度和取代基有关。
Six kinds of water-soluble chitosan were prepared by grafting aminofunctionality onto chitosan at the C-6 position, and their antimicrobial activities were investigated against three Gram-negative and three Gram-positive bacteria. Among the derivatives, dimethylaminoethyl-chitosan (DMAEC) has the highest potential to suppress the growth of bacteria. To elucidate detailed antimicrobial modes of action against bacteria, cell integrity, outer membrane (OM), and inner membrane (IM) permeabilization assays were investigated. When treated with DMAEC, the release of 260 nm absorbing materials quickly increased for both Escherichia coli and Staphylococcus aureus, but the absorbance value was different due to the difference in cell structures. In OM and IM permeabilization assays, DMAEC rapidly increased 1-N-phenylnaphthylamine uptake and the release of cytoplasmic beta-galactosidase via an increase in the permeability of OM and IM. Moreover, DMAEC90 prepared from 90% deacetylated chitosan had more activity than DMAEC50 prepared from 50% deacetylated chitosan, and these results revealed that the antimicrobial action of water-soluble chitosans was dependent on the degree of deacetylation and the substituted group.