Gene gun immunization in a preclinical model is enhanced by B7 targeting
Gene gun immunization in a preclinical model is enhanced by B7 targeting
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DOI：
10.1016/s0264-410x(03)00162-2
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发表时间：
2003-06-20
期刊：
影响因子：
5.5
通讯作者：
Andrew, ME
中科院分区：
文献类型：
--
作者：
Tachedjian, M;Boyle, JS;Andrew, ME
DNA疫苗具有巨大的潜力，但尽管在啮齿动物模型中显示出了希望，但在包括人类在内的大型动物中的反应令人失望。此外，DNA的基因枪递送已用于改善这些反应。然而，大多数被转染的细胞不是专职抗原呈递细胞（APC），其对于产生初次免疫应答是关键的。在这里，我们表明，在猪的大型动物模型中，基因枪递送和通过表达CTLA4-卵清蛋白（OVA）融合抗原靶向抗原呈递细胞的DNA载体的组合使用，导致卵清蛋白特异性血清IgG，IgA，IgG1和IgG2免疫应答增强。(C)2003爱思唯尔科技有限公司版权所有。
DNA vaccines have great potential but despite the promise shown in rodent models, responses in large animals, including humans, have been disappointing. Furthermore, gene gun delivery of DNA has been used to improve these responses. However, most cells that are transfected are not the professional antigen presenting cells (APC) which are critical for generating the primary immune response. Here, we show that in the large animal model of the pig, the combination of the use of gene gun delivery and a DNA vector that targets antigen presenting cells by expressing a CTLA4-ovalbumin (OVA) fusion antigen, leads to enhanced ovalbumin specific serum IgG, IgA, IgG1 and IgG2 immune responses. (C) 2003 Elsevier Science Ltd. All rights reserved.