NMR Techniques in Metabolomic Studies: A Quick Overview on Examples of Utilization.
NMR Techniques in Metabolomic Studies: A Quick Overview on Examples of Utilization.
复制标题
代谢组学研究中的NMR技术：有关利用示例的快速概述。
DOI：
10.1007/s00723-016-0846-9
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发表时间：
2017
影响因子：
1
通讯作者：
Kujawski J
中科院分区：
文献类型：
--
作者：
Kruk J;Doskocz M;Jodłowska E;Zacharzewska A;Łakomiec J;Czaja K;Kujawski J
代谢组学是一门快速发展的科学分支，专注于鉴定具有潜在生物标志物特性的生物活性分子。为了确定疾病的最佳生物标志物，代谢组学使用模型（体外，动物）和人类，以及各种技术，如质谱，气相色谱，液相色谱，红外和紫外-可见光谱和核磁共振。最后一种方法利用了某些原子核的磁性，如1H， 13C, 31P, 19F，特别是它们吸收和发射能量的能力，这对分析样品至关重要。在许多光谱核磁共振技术中，不仅已知一维（1D）技术，而且多年来还使用了二维（2D，例如COSY， DOSY， JRES， HETCORE， HMQS），三维（3D, DART-MS， HRMAS， HSQC， HMBC）和固态核磁共振。本文通过对核磁共振技术的基本划分，阐述了核磁共振技术在代谢组学研究特别是生物标志物鉴定中的广泛应用。
Metabolomics is a rapidly developing branch of science that concentrates on identifying biologically active molecules with potential biomarker properties. To define the best biomarkers for diseases, metabolomics uses both models (in vitro, animals) and human, as well as, various techniques such as mass spectroscopy, gas chromatography, liquid chromatography, infrared and UV–VIS spectroscopy and nuclear magnetic resonance. The last one takes advantage of the magnetic properties of certain nuclei, such as 1H, 13C, 31P, 19F, especially their ability to absorb and emit energy, what is crucial for analyzing samples. Among many spectroscopic NMR techniques not only one-dimensional (1D) techniques are known, but for many years two-dimensional (2D, for example, COSY, DOSY, JRES, HETCORE, HMQS), three-dimensional (3D, DART-MS, HRMAS, HSQC, HMBC) and solid-state NMR have been used. In this paper, authors taking apart fundamental division of nuclear magnetic resonance techniques intend to shown their wide application in metabolomic studies, especially in identifying biomarkers.